Detection of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) RNA in the Human Eye

被引：3

作者：

Kamalipour, Alireza ^{[1
]}

Ashraf, Mohammad Ali ^{[2
,3
]}

Moghimi, Sasan ^{[1
]}

Moattari, Afagh ^{[4
,5
]}

Ashraf, Mohammad Javad ^{[6
]}

Abbasi, Farhad ^{[7
]}

Azodi, Farzan ^{[8
]}

Oboudi, Shadi ^{[3
]}

Pirbonyeh, Neda ^{[4
,9
]}

Mokhtaryan, Maryam ^{[10
]}

Roshanshad, Amirhossein ^{[2
,3
]}

Do, Jiun L. ^{[1
]}

Weinreb, Robert N. ^{[1
]}

机构：

[1] Univ Calif San Diego, Shiley Eye Inst, Hamilton Glaucoma Ctr, Viterbi Family Dept Ophthalmol, San Diego, CA 92103 USA

[2] Shiraz Univ Med Sci, Poostchi Ophthalmol Res Ctr, Shiraz, Iran

[3] Shiraz Univ Med Sci, Student Res Comm, Shiraz, Iran

[4] Shiraz Univ Med Sci, Sch Med, Dept Virol & Bacteriol, Shiraz, Iran

[5] Shiraz Univ Med Sci, Bioinformat & Computat Biol Res Ctr, Shiraz, Iran

[6] Shiraz Univ Med Sci, Sch Med, Dept Pathol, Shiraz, Iran

[7] Bushehr Univ Med Sci, Dept Infect Dis, Bushehr, Iran

[8] Bushehr Univ Med Sci, Student Res Comm, Bushehr, Iran

[9] Shiraz Univ Med Sci, Burn & Wound Healing Res Ctr, Dept Microbiol, Shiraz, Iran

[10] Shiraz Univ Med Sci, Dept Internal Med, Shiraz, Iran

来源：

OCULAR IMMUNOLOGY AND INFLAMMATION | 2023年 / 31卷 / 01期

关键词：

COVID-19; RT-PCR; SARS-CoV-2; autopsy; conjunctiva; aqueous humor; vitreous humor; OPTIC NEURITIS; MORTALITY; RECEPTOR;

D O I：

10.1080/09273948.2021.1980810

中图分类号：

R77 [眼科学];

学科分类号：

100212 ;

摘要：

Purpose To determine the presence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA in postmortem ocular specimens of patients with severe COVID-19 disease. Patients and methods Postmortem conjunctival (28 samples), aqueous humor (30 samples) and vitreous humor (30 samples) specimens were obtained bilaterally from the eyes of 15 deceased COVID-19 patients within one hour of death. The presence of viral RNA was evaluated in samples using Real-time reverse transcriptase-polymerase chain reaction (RT-PCR). Results Positive RT-PCR SARS-COV-2 results were found in one conjunctival and 2 vitreous humor samples. All aqueous humor samples tested negative for the presence of SARS-COV-2 RNA. Of note, three positive samples were obtained from three different patients. The overall prevalence of positive RT-PCR ocular samples was 3.4% among all samples and 20% at the patient level. Conclusion SARS-CoV-2 RNA is detectable in postmortem conjunctival and vitreous humor samples of patients with severe COVID-19.

引用

页码：32 / 38

页数：7

共 47 条

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