Detecting prokaryote-specific gene and other bacterial signatures in thrombi from patients with acute ischemic stroke

被引：4

作者：

Wang, Xiaoke ^{[1
]}

Gao, Jie ^{[1
]}

Chen, Yantong ^{[2
,3
]}

Zhang, Xiaohao ^{[1
]}

Dai, Zhengze ^{[4
]}

Dai, Qiliang ^{[1
]}

Peng, Mengna ^{[1
]}

Xiao, Lulu ^{[1
]}

Jia, Xuerong ^{[1
]}

Cai, Haodi ^{[5
]}

Mou, Tao ^{[1
]}

Li, Xiang ^{[3
]}

Xu, Gelin ^{[1
,2
,3
]}

机构：

[1] Nanjing Univ, Med Sch, Affiliated Jinling Hosp, Dept Neurol, 305 East Zhongshan Rd, Nanjing 210002, Jiangsu, Peoples R China

[2] Shenzhen Second Peoples Hosp, Dept Neurol, Shenzhen, Guangdong, Peoples R China

[3] Shenzhen Univ, Affiliated Hosp 1, Dept Neurol, Shenzhen, Guangdong, Peoples R China

[4] Nanjing Med Univ, Affiliated Hosp 4, Dept Neurol, Nanjing, Jiangsu, Peoples R China

[5] Univ Hosp Med Ctr Hamburg Eppendorf, Dept Neurol, Martinistr 52, D-20245 Hamburg, Germany

来源：

THROMBOSIS JOURNAL | 2024年 / 22卷 / 01期

基金：

中国国家自然科学基金;

关键词：

Bacteria; Thrombus; Ischemic stroke; DNA; Endovascular treatment; GUT; INFLAMMATION; ALCOHOL; RISK; ATHEROSCLEROSIS; ASSOCIATION; MICROBIOTA; ARTERIAL; DISEASE;

D O I：

10.1186/s12959-024-00583-x

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Background and purposeMicrobial infection has been associated with thrombogenesis. This study aimed to detect bacterium-specific genes and other signatures in thrombi from patients with acute ischemic stroke and to relate these signatures to clinical characteristics.MethodsBlood samples were collected before thrombectomy procedures, and thrombus samples were obtained during the procedure. Identification and classification of bacteria in the samples were accomplished using 16 S rRNA gene sequencing. Bacterium-specific structures were observed with transmission electron microscopy. Bacterium-specific biomarkers were detected through immunohistochemical staining.Results16 S rRNA gene was detected in 32.1% of the thrombus samples from 81 patients. Bacillus (0.04% vs. 0.00046%, p = 0.003), Parabacteroides (0.20% vs. 0.09%, p = 0.029), Prevotella (1.57% vs. 0.38%, p = 0.010), Streptococcus (1.53% vs. 0.29%, p = 0.001), Romboutsia (0.18% vs. 0.0070%, p = 0.029), Corynebacterium (1.61% vs. 1.26%, p = 0.026) and Roseburia (0.53% vs. 0.05%, p = 0.005) exhibited significantly higher abundance in thrombi compared to arterial blood. Bacteria-like structures were observed in 22 (27.1%), while whole bacteria-like structures were observed in 7 (8.6%) thrombi under transmission electron microscopy. Immunohistochemical staining detected bacterium-specific monocyte/macrophage markers in 51 (63.0%) out of 81 thrombi. Logistic regression analysis indicated that alcohol consumption was associated with a higher bacteria burden in thrombi (odds ratio = 3.19; 95% CI, 1.10-9.27; p = 0.033).ConclusionBacterial signatures usually found in the oral cavity and digestive tract were detected in thrombi from patients with ischemic stroke. This suggests a potential involvement of bacterial infection in the development of thrombosis. Long-term alcohol consumption may potentially enhance this possibility.

引用

页数：9