Aerobic Training with Naringin Supplementation Improved Spatial Cognition via H2S Signaling Pathway in Alzheimer's Disease Model Rats

Objectives This study examined the effects of one-month naringin administration and exercise training on cognitive impairment and H2S signaling pathway in an Amyloid beta (A beta)-injected rat. Methods Rats were divided into four groups: control group; rats underwent A beta microinjection surgery, exercise group; rats underwent A beta microinjection surgery and trained by treadmill for four weeks, naringin group; rats underwent A beta microinjection surgery, and rats orally administrated 80 mg.kg(-1) naringin for four weeks, naringin+exercise group; rats underwent A beta microinjection surgery and were trained by treadmill for four weeks, and also, rats orally administrated 80 mg.kg(-1) naringin for four weeks. After one month of treatment, spatial learning and memory were measured, and then hippocampi were sampled. S-adenosylmethionine (SAM), cystathionine-beta-synthase (CBS), hydrogen sulfide (H2S) levels, and neuronal death were detected in the hippocampi of rats. Results Naringin and exercise improved spatial learning (latency time, P < .001) and memory (P < .001) in the Morris Water Maze test in A beta-injected rats compared with the control group. SAM (P < .01), CBS (P < .001), and H2S (P < .01) levels are increased in the naringin+exercise group compared with the control group. Conclusion The result of this study supports the effect of exercise and/or naringin to improve cognitive dysfunction and cell death through the production of H2S.