Effects of basic media and plant growth regulators on Direct-somatic embryogenesis induction in vegetative strains obtained from the tissue culture of digitalis purpurea

被引：0

作者：

AL-Oqab, Mohammed Ahmed ^{[1
]}

Zaid, Salim ^{[1
]}

Al-Ammouri, Youssef ^{[2
]}

机构：

[1] Damascus Univ, Fac Sci, Dept Plant Biol, Damascus, Syria

[2] Natl Commiss Biotechnol, Damascus, Syria

来源：

EMIRATES JOURNAL OF FOOD AND AGRICULTURE | 2023年 / 35卷 / 02期

关键词：

Digitalis purpurea; Vegetative strains; Direct -somatic embryogenesis; Explant; Basic media; IN-VITRO REGENERATION; LEAF EXPLANTS; LANATA EHRH; L; ORGANOGENESIS; REQUIREMENTS; PHALAENOPSIS; PROPAGATION; CYTOKININS; FOXGLOVE;

D O I：

10.9755/ejfa.2023.v35.i2.3008

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

This study aimed to investigate the effects of certain nutrient medium types enhanced with plant-growth regulators on the direct induction of somatic embryogenesis in vegetative strains produced and propagated from a tissue culture of Digitalis purpurea. The results show that MS medium was superior to other tested nutrient media-B5, LS, and 5C01-in terms of the days required to initiate direct somatic embryogenesis induction and induction rate. In contrast, the LS medium resulted in the highest number of embryos. Moreover, 2 mg/L BA (benzyl adenine) was superior to similar concentrations of BAP (benzyl aminopurine) and Kin (kinetin) in the MS medium, whereas BAP performed better in LS and B5. On the other hand, auxin 2.4D (dichlorophenoxy acetic acid) inhibited somatic embryogenesis, as did NAA (naphthalene acetic acid) and IAA (indole acetic acid) when added individually. Regarding the type of explant, the stems showed the induction rates and numbers of direct-somatic embryos produced in all the basic media.

引用

页码：106 / 120

页数：15

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