Passive Recording of Bioelectrical Signals from Non-Excitable Cells by Fluorescent Mirroring

被引:5
作者
Moreddu, Rosalia [1 ]
Boschi, Alessio [1 ]
d'Amora, Marta [1 ]
Hubarevich, Aliaksandr [1 ]
Angelis, Francesco De [1 ]
机构
[1] Ist Italiano Tecnol, Plasmon Nanotechnol Unit, I-16163 Genoa, Italy
基金
欧盟地平线“2020”;
关键词
bioelectricity; non-excitable cells; HEK-293; cell-surface adhesion; fluorescence; POTENTIALS; BEHAVIOR; GROWTH; VIRUS;
D O I
10.1021/acs.nanolett.2c05053
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Bioelectrical variations trigger different cell responses, including migration, mitosis, and mutation. At the tissue level, these actions result in phenomena such as wound healing, proliferation, and pathogenesis. Monitoring these mechanisms dynamically is highly desirable in diagnostics and drug testing. However, existing technologies are invasive: either they require physical access to the intracellular compartments, or they imply direct contact with the cellular medium. Here, we present a novel approach for the passive recording of electrical signals from non-excitable cells adhering to 3D microelectrodes, based on optical mirroring. Preliminary results yielded a fluorescence intensity output increase of the 5,8% in the presence of a HEK-293 cell on the electrode compared to bare microelectrodes. At present, this technology may be employed to evaluate cell???substrate adhesion and monitor cell proliferation. Further refinements could allow extrapolating quantitative data on surface charges and resting potential to investigate the electrical phenomena involved in cell migration and cancer progression.
引用
收藏
页码:3217 / 3223
页数:7
相关论文
共 37 条
  • [31] Enteropathogenic Escherichia coli markedly decreases the resting membrane potential of Caco-2 and HeLa human epithelial cells
    Stein, MA
    Mathers, DA
    Yan, H
    Baimbridge, KG
    Finlay, BB
    [J]. INFECTION AND IMMUNITY, 1996, 64 (11) : 4820 - 4825
  • [32] Electrically induced bacterial membrane-potential dynamics correspond to cellular proliferation capacity
    Stratford, James P.
    Edwards, Conor L. A.
    Ghanshyam, Manjari J.
    Malyshev, Dmitry
    Delise, Marco A.
    Hayashi, Yoshikatsu
    Asally, Munehiro
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2019, 116 (19) : 9552 - 9557
  • [33] Thomas Philip, 2005, Journal of Pharmacological and Toxicological Methods, V51, P187, DOI 10.1016/j.vascn.2004.08.014
  • [34] /Nature's Electric Potential: A Systematic Review of the Role of Bioelectricity in Wound Healing and Regenerative Processes in Animals, Humans, and Plants
    Tyler, Sheena E. B.
    [J]. FRONTIERS IN PHYSIOLOGY, 2017, 8
  • [35] Ionic Components of Electric Current at Rat Corneal Wounds
    Vieira, Ana Carolina
    Reid, Brian
    Cao, Lin
    Mannis, Mark J.
    Schwab, Ivan R.
    Zhao, Min
    [J]. PLOS ONE, 2011, 6 (02):
  • [36] Bioelectricity, Its Fundamentals, Characterization Methodology, and Applications in Nano-Bioprobing and Cancer Diagnosis
    Wang, Yilong
    Han, Xiao
    Cui, Zheng
    Shi, Donglu
    [J]. ADVANCED BIOSYSTEMS, 2019, 3 (10)
  • [37] Electrical signals control wound healing through phosphatidylinositol-3-OH kinase-γ and PTEN
    Zhao, Min
    Song, Bing
    Pu, Jin
    Wada, Teiji
    Reid, Brian
    Tai, Guangping
    Wang, Fei
    Guo, Aihua
    Walczysko, Petr
    Gu, Yu
    Sasaki, Takehiko
    Suzuki, Akira
    Forrester, John V.
    Bourne, Henry R.
    Devreotes, Peter N.
    McCaig, Colin D.
    Penninger, Josef M.
    [J]. NATURE, 2006, 442 (7101) : 457 - 460