QTL Mapping of Zeaxanthin Content in Sweet Corn Using Recombinant Inbred Line Population across Different Environments

被引:2
作者
Zhang, Yahui [1 ]
Tang, Yunqi [1 ]
Jin, Weicai [1 ]
Liu, Yu [1 ]
Li, Guangyu [1 ]
Zhong, Wenhao [1 ]
Huang, Jun [1 ]
Wang, Wenyi [1 ]
机构
[1] South China Agr Univ, Coll Agr, Guangzhou 510642, Peoples R China
来源
PLANTS-BASEL | 2023年 / 12卷 / 19期
关键词
RIL population; zeaxanthin; QTL mapping; sweet corn; LOD; QUANTITATIVE TRAIT LOCI; MACULAR DEGENERATION; CAROTENOID CONTENTS; PROTECTIVE ROLE; ACCUMULATION; LUTEIN; IDENTIFICATION; HYDROXYLASE; PROGRESSION; IMPAIRMENT;
D O I
10.3390/plants12193506
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Zeaxanthin is a naturally occurring xanthophyll carotenoid obtained from diet sources. Particularly, sweet corn is a major source of dietary zeaxanthin. To investigate the genetic basis of zeaxanthin content regulation in sweet corn, a recombinant inbred line (RIL) population comprising 191 families was constructed using two inbred lines (K44 and F22) with contrasting zeaxanthin content in the grain. The zeaxanthin content in the dry grains of this population grown at different locations was determined using high performance liquid chromatography (HPLC). Subsequently, 175 polymorphic simple sequence repeat (SSR) markers were used to construct a linkage map with a total length of 4322.37 cM and with an average distance of 24.4 cM. A total of eight QTLs located on chromosomes 4, 5, 7, 9, and 10 were detected. The QTLs located in umc1632-umc1401 on chromosome 7 were detected in different environments and explained 11.28-20.25% of the phenotypic variation, implying it is the main QTL controlling zeaxanthin content in the dry grains of sweet corn. Collectively, the present study provides a genetic map and theoretical guidance for the cultivation of sweet corn varieties with a high zeaxanthin content.
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页数:12
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共 51 条
[1]   What do we know about the macular pigment in AMD: the past, the present, and the future [J].
Arunkumar, Ranganathan ;
Calvo, Charles M. ;
Conrady, Christopher D. ;
Bernstein, Paul S. .
EYE, 2018, 32 (05) :992-1004
[2]   Genomics-Integrated Breeding for Carotenoids and Folates in Staple Cereal Grains to Reduce Malnutrition [J].
Ashokkumar, Kaliyaperumal ;
Govindaraj, Mahalingam ;
Karthikeyan, Adhimoolam ;
Shobhana, V. G. ;
Warkentin, Thomas D. .
FRONTIERS IN GENETICS, 2020, 11
[3]  
Ashrafi H, 2009, GENOME, V52, P935, DOI [10.1139/G09-065, 10.1139/g09-065]
[4]  
BONE RA, 1993, INVEST OPHTH VIS SCI, V34, P2033
[5]   Absorption, metabolism, and functions of β-cryptoxanthin [J].
Burri, Betty J. ;
La Frano, Michael R. ;
Zhu, Chenghao .
NUTRITION REVIEWS, 2016, 74 (02) :69-82
[6]   Using molecular markers to identify two major loci controlling carotenoid contents in maize grain [J].
Chander, Subhash ;
Guo, Y. Q. ;
Yang, X. H. ;
Zhang, J. ;
Lu, X. Q. ;
Yan, J. B. ;
Song, T. M. ;
Rocheford, T. R. ;
Li, J. S. .
THEORETICAL AND APPLIED GENETICS, 2008, 116 (02) :223-233
[7]   Secondary Analyses of the Effects of Lutein/Zeaxanthin on Age-Related Macular Degeneration Progression AREDS2 Report No. 3 [J].
Chew, Emily Y. ;
Clemons, Traci E. ;
SanGiovanni, John Paul ;
Danis, Ronald P. ;
Ferris, Frederick L. ;
Elman, Michael J. ;
Antoszyk, Andrew N. ;
Ruby, Alan J. ;
Orth, David ;
Bressler, Susan B. ;
Fish, Gary E. ;
Hubbard, George Baker ;
Klein, Michael L. ;
Chandra, Suresh R. ;
Blodi, Barbara A. ;
Domalpally, Amitha ;
Friberg, Thomas ;
Wong, Wai T. ;
Rosenfeld, Philip J. ;
Agron, Elvira ;
Toth, Cynthia A. ;
Bernstein, Paul S. ;
Sperduto, Robert D. .
JAMA OPHTHALMOLOGY, 2014, 132 (02) :142-149
[8]  
Congdon N, 2004, ARCH OPHTHALMOL-CHIC, V122, P477
[9]   Overexpression of CrtR-b2 (carotene beta hydroxylase 2) from S. lycopersicum L. differentially affects xanthophyll synthesis and accumulation in transgenic tomato plants [J].
D'Ambrosio C. ;
Stigliani A.L. ;
Giorio G. .
Transgenic Research, 2011, 20 (1) :47-60
[10]   Metabolic engineering of xanthophyll content in tomato fruits [J].
Dharmapuri, S ;
Rosati, C ;
Pallara, P ;
Aquilani, R ;
Bouvier, F ;
Camara, B ;
Giuliano, G .
FEBS LETTERS, 2002, 519 (1-3) :30-34