Beneficial effects of topical 6-gingerol loaded nanoemulsion gel for wound and inflammation management with their comparative dermatokinetic

Purpose: To develop, formulate and evaluate a 6-Gingerol (GL) loaded self nano emulsifying drug delivery system and its conversion to nanoemulgel to achieve better topical application via their solubility improvement as well as permeation of skin for the management of wound healing and anti-inflammatory mechanism.Methods: We used the aqueous microtitration method for the preparation of GL-NE with the help of Oleic Acid (oil phase), Tween 20 (surfactant), and PEG-400 (co-surfactant). To improve contact time for topical delivery, it was loaded into Carbopol 934 gel to form nanoemulgel. Nanoemulsion and nanoemulgel were evaluated for globule size, thermodynamic stability, physical appearance, ZP, PDI, pH, drug content, rheological behavior, drug release (in-vitro) with skin permeation, spreadability, DSC, swelling index and bioadhesive strength.Results: GL-SN7 showed a clear, stable, and transparent nanoemulsion containing mean droplet size (91.36 +/- 4.61 nm), zeta potential (-9.12 +/- 0.79 mV), and PDI (0.119 +/- 0.005). Nanoemulgel (NEG) showed that showed the mean droplet size (103.24 +/- 6.31 nm) and PDI (0.126 +/- 0.123), and ZP (-10.8 +/- 0.95 mV), pH (6.69 +/- 0.06), viscosity (5552 cP) at 100 rpm and spreadability 234.10 +/- 0.75 g cm/sec with better mucoadhesive strength with their smooth and spherical shape by SEM. Finally, optimized-6-GL-SNEDDS-Gel was used to enhance skin permeation and their dermatokinetic results showed a significant (p<0.001) enhancement in the CSkin max and AUC0-8h in the treated skin with optimized-6-GL-SNEDDS-Gel as compared to 6-GL-conventionalgel treated with skin. However, optimized-6-GL-SNEDDS-Gel found significant wound healing as compared to GL-S on topical application. We didn't observe any signs of inflammatory cells after the treatment from the GLSNEDDS-Gel as well as suggested the safety and non-toxicity of optimized-GL-SNEDDS-Gel.Conclusion: We have successfully developed a novel GL-SNEDDS-Gel. It improved the solubility and skin permeation of GL. GL-SNEDDS-Gel showed a better treatment of wound healing as well as anti-inflammatory treatment of GL given topically.