Identification of coniferyl ferulate as the bioactive compound behind the xanthine oxidase inhibitory activity of Chuanxiong Rhizome

被引：10

作者：

Wang, Hui ^{[1
]}

Zhang, Hongwei ^{[2
]}

Zhang, Xiaomei ^{[2
]}

Yin, Yichen ^{[1
]}

Ding, Guiquan ^{[1
]}

Tang, Xiaowen ^{[1
]}

Hou, Pengyi ^{[3
]}

Sun, Shiwei ^{[1
]}

Wang, Wei ^{[1
]}

机构：

[1] Qingdao Univ, Sch Pharm, Dept Nat Med & Pharmacognosy, 1 Ningde Rd, Qingdao 266071, Peoples R China

[2] Technol Ctr Qingdao Customs Dist, Qingdao 266005, Peoples R China

[3] Shanghai AB Sciex Analyt Instrument Trading Co Ltd, Beijing 100015, Peoples R China

来源：

JOURNAL OF FUNCTIONAL FOODS | 2023年 / 100卷

关键词：

Coniferyl ferulate; Xanthine oxidase inhibitor; UPLC-ZenoTOF; Isobologram; Fluorescence quenching; Molecular docking; ALLOPURINOL; COMPONENTS; PLASMA;

D O I：

10.1016/j.jff.2022.105378

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Xanthine oxidase (XO) has been well-recognized as a key enzyme in the pathogenesis of hyperuricemia. In this study, Chuanxiong Rhizome, a tonic food widely consumed in China, was found to inhibit XO activity. The potential XO inhibitory ingredients were screened by UPLC-ZenoTOF system and bioactivity assays. Coniferyl ferulate (CF), a main constitute of Chuanxiong Rhizome, was identified as a potent XO inhibitor with an IC50 value (1.97 +/- 0.11 mu M) lower than that of allopurinol (2.49 +/- 0.07 mu M). Enzyme-kinetic assays showed that CF reversibly inhibited XO in an uncompetitive manner. And hydrogen bonds played crucial roles on the binding interaction according to the fluorescence titration and molecular docking. Moreover, the combination of CF with allopurinol exhibited a synergistic inhibitory effect on XO. Our results revealed for the first time the XO inhibitory effect of CF from Chuanxiong Rhizome, which shed light on its potential application for developing anti-hyperuricemia agents.

引用

页数：8

共 38 条

[1] Coniferyl Ferulate, a Strong Inhibitor of Glutathione S-Transferase Isolated from Radix Angelicae sinensis, Reverses Multidrug Resistance and Downregulates P-Glycoprotein [J].

Chen, Chang ;

Wu, Chuanhong ;

Lu, Xinhua ;

Yan, Zhiyong ;

Gao, Jian ;

Zhao, Hui ;

Li, Shaojing .

EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE, 2013, 2013

[2] Synthesis and biological evaluation of geniposide derivatives as inhibitors of hyperuricemia, inflammatory and fibrosis [J].

Chen, Jia-shu ;

Wang, Mu-xuan ;

Wang, Min-min ;

Zhang, Yu-kai ;

Guo, Xu ;

Chen, Ying-ying ;

Zhang, Meng-qi ;

Sun, Jin-yue ;

Liu, Yu-fa ;

Liu, Chao .

EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY, 2022, 237

[3] A systematic review on the rhizome of Ligusticum chuanxiong Hort. (Chuanxiong) [J].

Chen, Zhejie ;

Zhang, Chen ;

Gao, Fei ;

Fu, Qiang ;

Fu, Chaomei ;

He, Yao ;

Zhang, Jinming .

FOOD AND CHEMICAL TOXICOLOGY, 2018, 119 :309-325

[4] Antibacterial activity of components from Lomatium californicum [J].

Chou, SC ;

Everngam, MC ;

Sturtz, G ;

Beck, JJ .

PHYTOTHERAPY RESEARCH, 2006, 20 (02) :153-156

[5] Theoretical basis, experimental design, and computerized simulation of synergism and antagonism in drug combination studies [J].

Chou, Ting-Chao .

PHARMACOLOGICAL REVIEWS, 2006, 58 (03) :621-681

[6] Control of Plasma Uric Acid in Adults at Risk for Tumor Lysis Syndrome: Efficacy and Safety of Rasburicase Alone and Rasburicase Followed by Allopurinol Compared With Allopurinol Alone-Results of a Multicenter Phase III Study [J].

Cortes, Jorge ;

Moore, Joseph O. ;

Maziarz, Richard T. ;

Wetzler, Meir ;

Craig, Michael ;

Matous, Jeffrey ;

Luger, Selina ;

Dey, Bimalangshu R. ;

Schiller, Gary J. ;

Pham, Dat ;

Abboud, Camille N. ;

Krishnamurthy, Muthuswamy ;

Brown, Archie, Jr. ;

Laadem, Abderrahmane ;

Seiter, Karen .

JOURNAL OF CLINICAL ONCOLOGY, 2010, 28 (27) :4207-4213

[7] U-shaped association of uric acid to overall-cause mortality and its impact on clinical management of hyperuricemia [J].

Crawley, William T. ;

Jungels, Cyprien G. ;

Stenmark, Kurt R. ;

Fini, Mehdi A. .

REDOX BIOLOGY, 2022, 51

[8] Discovery of mycotoxin alternariol as a potential lead compound targeting xanthine oxidase [J].

Fan, Jiahe ;

Sun, Shiwei ;

Lv, Chaoyi ;

Li, Zhizhou ;

Guo, Mengru ;

Yin, Yichen ;

Wang, Hui ;

Wang, Wei .

CHEMICO-BIOLOGICAL INTERACTIONS, 2022, 360

[9] Synthesis, molecular docking and xanthine oxidase inhibitory activity of 5-aryl-1H-tetrazoles [J].

Fatima, Itrat ;

Zafar, Humaira ;

Khan, Khalid Mohammed ;

Saad, Syed Muhammad ;

Javaid, Sumaira ;

Perveen, Shahnaz ;

Choudhary, M. Iqbal .

BIOORGANIC CHEMISTRY, 2018, 79 :201-211

[10]

Gao Xin, 2018, Northwest Pharmaceutical Journal, V33, P711

← 1 2 3 4 →