A novel biomimetic network amplification strategy designed fluorescent aptasensor based on yolk-shell Fe3O4 nanomaterials for aflatoxin B1 detection

被引：10

作者：

Dong, Xiaoze ^{[1
]}

Qi, Shuo ^{[1
]}

Qin, Mingwei ^{[1
]}

Sun, Yuhan ^{[1
]}

Lv, Yan ^{[1
]}

Zhang, Yin ^{[3
]}

Wang, Zhouping ^{[1
,2
,3
,4
,5
]}

机构：

[1] Jiangnan Univ, State Key Lab Food Sci & Technol, Int Joint Lab Food Safety, Wuxi 214122, Peoples R China

[2] Jiangnan Univ, Sch Food Sci & Technol, Wuxi 214122, Peoples R China

[3] Chengdu Univ, Key Lab Meat Proc Sichuan, Chengdu 610106, Peoples R China

[4] Jiangnan Univ, Natl Engn Res Ctr Funct Food, Wuxi 214122, Peoples R China

[5] Jiangnan Univ, Collaborat Innovat Ctr Food Safety & Qual Control, Food, Wuxi 214122, Peoples R China

来源：

FOOD CHEMISTRY | 2023年 / 398卷

关键词：

AFB1; Aptamer; Yolk-shell; Fe3O4; Constitutional dynamic networks; APTAMER; ASSAY; BIOSENSOR; PLATFORM; POLYMER;

D O I：

10.1016/j.foodchem.2022.133761

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

In this study, yolk-shell Fe3O4-based nanomaterials designing of fluorescent aptasensor using a new type of biomimetic network-constitutional dynamic networks (CDNs) amplification strategy was developed for aflatoxin B1 (AFB1) detection. By ingeniously designing the base sequences A, a, B, b, and coupling with endonuclease (BbvCI), a constitutes of CDNs is formed as a fluorescence intensity amplifier. With such design, the as-prepared aptasensor exhibits good sensitivity from 50 fg & BULL;mL-1-50 ng & BULL;mL-1 with a detection limit of 35.94 fg & BULL;mL-1. Moreover, the CDNs can achieve 1000-fold amplification in terms of linear range and detection limit. The results confirmed that this new type of biomimetic network amplification strategy can supply-one efficient approach to improve signal amplification. Furthermore, the prepared aptamer sensor was tested for spiked recovery in peanut samples, and the recoveries ranged from 83.79 to 95.06 %, which has confirmed its practical application value in the field of food safety.

引用

页数：8

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