Exogenous melatonin mitigates chilling injury in zucchini fruit by enhancing antioxidant system activity, promoting endogenous proline and GABA accumulation, and preserving cell wall stability

被引：36
作者：
Ali, Sajid [1 ]
Nawaz, Aamir [1 ]
Naz, Safina [1 ]
Ali, Maratab [2 ,3 ]
Ejaz, Shaghef [1 ]
Azam, Muhammad [4 ]
Razzaq, Kashif [5 ]
机构：
[1] Bahauddin Zakariya Univ, Fac Agr Sci & Technol, Dept Hort, Multan 60800, Pakistan
[2] Shandong Univ Technol, Coll Agr Engn & Food Sci, Zibo 255000, Shandong, Peoples R China
[3] Univ Management & Technol, Sch Food & Agr Sci, Lahore 54000, Punjab Province, Pakistan
[4] Univ Agr Faisalabad, Inst Hort Sci, Fac Agr, Pomol Lab, Faisalabad 38040, Pakistan
[5] MNS Univ Agr, Dept Hort, Old Shujabad Rd, Multan, Pakistan
来源：
POSTHARVEST BIOLOGY AND TECHNOLOGY | 2023年 / 204卷
关键词：
Antioxidant activity; Cell wall degradation; Chilling stress; GABA shunt; Proline metabolism; GAMMA-AMINOBUTYRIC-ACID; PEACH FRUIT; TREATMENT INCREASES; PROTECTIVE ROLE; COLD-STORAGE; NITRIC-OXIDE; METABOLISM; TOLERANCE; QUALITY; SHUNT;
D O I：
10.1016/j.postharvbio.2023.112445
中图分类号：
S3 [农学（农艺学）];
学科分类号：
0901 ;
摘要：
Zucchini fruit are sensitive to chilling injury (CI) during postharvest low temperature storage which makes their cold stored life short. In this work, zucchini fruit were treated with melatonin (200 & mu;mol L-1) and stored at 5 oC for 15 d. The pre-storage melatonin treatment reduced weight loss (6.51%) and suppressed symptoms of CI (1.5 Score) along with lower malondialdehyde (MDA), relative ion leakage (RIL), superoxide anion and hydrogen peroxide (H2O2) in contrast with control. The treated zucchini exhibited higher L* (51.89) and lower a* (-10.17) and b* (29.15) values of exocarp in comparison with control. Melatonin treated zucchini showed enhanced & gamma;-aminobutyric acid (GABA) concentration due to higher glutamate content and stimulated glutamate decarboxylase (GAD) and suppressed & gamma;-aminobutyric acid transaminase (GABA-T) activities. In addition, melatonin application increased proline accumulation owing to stimulation of & UDelta;-1-pyrroline-5-carboxylate synthetase (P5CS) and ornithine & delta;-aminotransferase (OAT) enzymes and reduced proline dehydrogenase (ProDH) activity as compared with control. The activity of antioxidative such as ascorbate peroxidase (APX), peroxidase (POD), catalase (CAT) and superoxide dismutase (SOD) enzymes remained higher in melatonin treated zucchini. The fruit treated with melatonin exhibited reduced water soluble pectin (WSPN) along with higher sodium carbonate soluble pectin (SCSP), protopectin (PRPN), chelate soluble pectin (CSPN), cellulose (CLS) and hemicellulose (HLS) in comparison with control. The exogenous melatonin suppressed the activities of polygalacturonase (PG), & beta;-galactosidase (& beta;-Gal), cellulase (CX), and pectin methylesterase (PME) along with higher firmness. In conclusion, exogenous melatonin at 200 & mu;mol L-1 concentration could be used for zucchini fruit CI mitigation under cold storage.
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页数：17
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