A non-viral gene therapy for melanoma by staphylococcal enterotoxin A

被引:2
|
作者
Yang, Ling [1 ,2 ]
Ren, Min [1 ,2 ]
Wang, Jie [1 ,2 ]
He, Liming [1 ,2 ]
Wu, Shanshan [3 ]
Yang, Shuai [1 ,2 ]
Zhao, Wei [1 ,2 ]
Cheng, Hao [1 ,2 ]
Zhou, Xiaoming [4 ]
Gou, Maling [1 ,2 ]
机构
[1] Sichuan Univ, State Key Lab Biotherapy, Chengdu 610041, Peoples R China
[2] Sichuan Univ, West China Hosp, Canc Ctr, Chengdu 610041, Peoples R China
[3] Sichuan Univ, West China Hosp, Dept Ophthalmol, Chengdu 610041, Peoples R China
[4] Sichuan Univ, West China Hosp, Dept Integrated Tradit Chinese & Western Med, Rare Dis Ctr,State Key Lab Biotherapy, Chengdu 610041, Peoples R China
关键词
Gene therapy; Superantigen; Melanoma; Staphylococcal enterotoxins A; Immunotherapyene therapy; IMMUNE CHECKPOINT INHIBITORS;
D O I
10.1016/j.cclet.2023.108822
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Staphylococcal enterotoxin A (SEA) derived from Staphylococcus aureus , as a superantigen, shows potential for cancer immunotherapy, but systemic immunotoxicity restricts its clinical application. Targeted delivery of SEA to tumor site provides a promising option for reducing the systemic toxicity. Here, we constructed an iRGD peptide (H-[Cys-Arg-Gly-Asp-Lys-Gly-Pro-Asp-Cys]-NH2 ) modified nanoparticle (iDPP) to deliver plasmids encoding SEA for melanoma treatment. The iDPP/SEA nanocomplexes efficiently mediated SEA expression in B16-F10 cells in vivo and in vitro and induced the activation of lymphocytes and maturation of murine bone marrow-derived dendritic cells (BMDCs) in vitro . In the subcutaneous B16-F10 melanoma model, the iDPP/SEA nanocomplexes could effectively enhance immune response and T lymphocytes infiltration in tumor site after intravenous administration, thereby considerably decreased melanoma growth. Meanwhile, no obvious adverse effect was observed after intravenous administration of the iDPP/SEA nanocomplexes in vivo . Our findings demonstrated that gene therapy of SEA is a potential candidate for melanoma treatment. (c) 2024 Published by Elsevier B.V. on behalf of Chinese Chemical Society and Institute of Materia Medica, Chinese Academy of Medical Sciences.
引用
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页数:5
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