Cryo-EM structure of the cytosolic AhR complex

被引：30

作者：

Wen, Zuoling ^{[1
,2
]}

Zhang, Yuebin ^{[3
]}

Zhang, Beirong ^{[2
,4
]}

Hang, Yumo ^{[5
]}

Xu, Li ^{[6
]}

Chen, Yangsheng ^{[6
]}

Xie, Qunhui ^{[6
]}

Zhao, Qun ^{[4
]}

Zhang, Lihua ^{[4
]}

Li, Guohui ^{[3
]}

Zhao, Bin ^{[6
]}

Sun, Fei ^{[1
,2
,7
]}

Zhai, Yujia ^{[1
]}

Zhu, Yun ^{[1
]}

机构：

[1] Chinese Acad Sci, Inst Biophys, CAS Ctr Excellence Biomacromol, Natl Key Lab Biomacromol, Beijing 100101, Peoples R China

[2] Univ Chinese Acad Sci, Beijing, Peoples R China

[3] Chinese Acad Sci, Dalian Inst Chem Phys, State Key Lab Mol React Dynam, Dalian, Peoples R China

[4] Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Liaoning, Peoples R China

[5] Huazhong Univ Sci & Technol, Tongji Med Coll, Sch Basic Med, Dept Pathogen Biol, Wuhan 430030, Peoples R China

[6] Chinese Acad Sci, Res Ctr Ecoenvironm Sci, State Key Lab Environm Chem & Ecotoxicol, Beijing 100085, Peoples R China

[7] Chinese Acad Sci, Inst Biophys, Ctr Biol Imaging, Core Facil Prot Sci, Beijing, Peoples R China

来源：

STRUCTURE | 2023年 / 31卷 / 03期

关键词：

LIGAND-BINDING; PROTEIN INTERACTION; PAS DOMAINS; RECEPTOR; DIMERIZATION; IDENTIFICATION; RECOGNITION; MECHANISM; REVEALS; MICE;

D O I：

10.1016/j.str.2022.12.013

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Aryl hydrocarbon receptor (AhR) is an important ligand-activated transcription factor involved in the regula-tion of various important physiological functions. Here, we report the cryo-EM structures of the Hsp90-AhR-p23 complex with or without bound XAP2, where the structure of the mouse AhR PAS-B domain is resolved. A highly conserved bridge motif of AhR is responsible for the interaction with the Hsp90 dimeric lumen. The ligand-free AhR PAS-B domain is attached to the Hsp90 dimer and is stabilized in the complex with bound XAP2. In addition, the DE-loop and a group of conserved pocket inner residues in the AhR PAS-B domain are found to be important for ligand binding. These results reveal the structural basis of the biological func-tions of AhR. Moreover, the protein purification method presented here allows the isolation of stable mouse AhR protein, which could be used to develop high-sensitivity biosensors for environmental pollutant detection.

引用

页码：295 / +

页数：19

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