Evolutionary and Genetic Recombination Analyses of Coxsackievirus A6 Variants Associated with Hand, Foot, and Mouth Disease Outbreaks in Thailand between 2019 and 2022

被引:10
作者
Puenpa, Jiratchaya [1 ]
Saengdao, Nutsada [1 ,2 ]
Khanarat, Nongkanok [1 ]
Korkong, Sumeth [1 ]
Chansaenroj, Jira [1 ]
Yorsaeng, Ritthideach [1 ]
Wanlapakorn, Nasamon [1 ]
Poovorawan, Yong [1 ,3 ]
机构
[1] Chulalongkorn Univ, Fac Med, Ctr Excellence Clin Virol, Dept Pediat, Bangkok 10330, Thailand
[2] Mahidol Univ, Siriraj Hosp, Fac Med, Dept Microbiol, Bangkok 10700, Thailand
[3] Royal Soc Thailand, FRS T, Sanam Sueapa, Dusit, Bangkok 10300, Thailand
来源
VIRUSES-BASEL | 2023年 / 15卷 / 01期
关键词
coxsackievirus A6; hand; foot; and mouth disease; evolution; complete genome sequencing; genetic recombination; ENTEROVIRUS; 71; ATYPICAL HAND; MOLECULAR EPIDEMIOLOGY; CHINA; A16; EMERGENCE; CHILDREN;
D O I
10.3390/v15010073
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Coxsackievirus (CV)-A6 infections cause hand, foot, and mouth disease (HFMD) in children and adults. Despite the serious public health threat presented by CV-A6 infections, our understanding of the mechanisms by which new CV-A6 strains emerge remains limited. This study investigated the molecular epidemiological trends, evolutionary dynamics, and recombination characteristics of CV-A6-associated HFMD in Thailand between 2019 and 2022. In the HFMD patient samples collected during the 4-year study period, we identified enterovirus (EV) RNA in 368 samples (48.7%), of which CV-A6 (23.7%) was the predominant genotype, followed by CV-A4 (6%), EV-A71 (3.7%), and CV-A16 (3.4%). According to the partial viral protein (VP) 1 sequences, all these CV-A6 strains belonged to the D3 clade. Based on the viral-RNA-dependent RNA polymerase (RdRp) gene, four recombinant forms (RFs), RF-A (147, 84.5%), RF-N (11, 6.3%), RF-H (1, 0.6%), and newly RF-Y (15, 8.6%), were identified throughout the study period. Results from the similarity plot and bootscan analyses revealed that the 3D polymerase (3Dpol) region of the D3/RF-Y subclade consists of sequences highly similar to CV-A10. We envisage that the epidemiological and evolutionarily insights presented in this manuscript will contribute to the development of vaccines to prevent the spread of CV-A6 infection.
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页数:14
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