Antibacterial efficacy of NanoCare, Fullerene (C60) activated by UV light, and Morinda Oleifera against S.Mutans and bond integrity of composite resin to caries affected dentin

Aim: Assessment of the impact of contemporary disinfection techniques Moringa Oleifera (M. Oleifera), Nano Care Plus Silver Gold (R) (Nanocare), and Fullerene (C60) on survival rates of S.Mutans and shear bond strength (SBS) of composite to the carious affected dentin (CAD) Material and methods: Sixty mandibular molars having caries progression till the middle 1/3rd of the dentin were disinfected. The CAD surface of twenty samples was inoculated with S.mutans. Based on different disinfection regimens samples were arbitrarily assigned to four groups (n:15) Group 1: 2 % CHX, Group 2:NanoCare, Group 3: Fullerene (C60), and Group 4 (M. Oleifera. This was followed by calculating the survival rate of S.mutans. Ten samples from each group were then restored with composite restoration and thermocycled. Assessment of SBS and failure mode was performed using a universal testing machine and stereomicroscope at 40X magnification. Statistical significance among groups was assessed using analysis of variance (ANOVA) and Tukey's test at a significance level of p = 0.05 Results: Group 2 samples treated with NanoCare exhibited the lowest survival rate (0.39 +/- 0.02 CFU/ml) of S. Mutans. However, Group 1 (CHX) samples exhibited the highest survival count (0.51 +/- 0.10 CFU/ml). Furthermore, the highest composite to CAD bond was observed in Group 3 Fullerene(C60) (18.44 +/- 0.25 MPa) samples and the lowest SBS was observed in Group 1 (CHX) (12.48 +/- 1.69 MPa) CONCLUSION: Fullerene(C60) and Moringa Oleifera extract hold promise as potential substitutes for chlorhexidine (CHX) in clinical applications, offering the potential for improved S.Mutans elimination and enhanced bond strength to CAD surface