Chlamydia trachomatis infection regulates the expression of tetraspanins, activin-A, and inhibin-A in tubal ectopic pregnancy

被引：2

作者：

Pant, Shipra ^{[1
]}

Bhati, Tanu ^{[1
]}

Dimri, Astha

Arora, Renu ^{[2
]}

Siraj, Fouzia ^{[3
]}

Raisuddin, Sheikh ^{[4
]}

Rastogi, Sangita ^{[1
]}

机构：

[1] ICMR Natl Inst Pathol, Mol Microbiol Lab, Sriramachari Bhawan, Safdarjung Hosp Campus,Post Box 4909, New Delhi 110029, India

[2] Vardhman Mahavir Med Coll VMMC & Safdarjung Hosp, Dept Obstet & Gynaecol, New Delhi 110029, India

[3] ICMR, Natl Inst Pathol, Pathol Lab, Safdarjung Hosp Campus,Post Box 4909, New Delhi 110029, India

[4] Jamia Hamdard, Dept Med Elementol & Toxicol, New Delhi 10062, India

来源：

PATHOGENS AND DISEASE | 2023年 / 81卷

关键词：

Chlamydia trachomatis; tubal ectopic pregnancy; Tspan5; CD63; activin-A; inhibin-A; FALLOPIAN-TUBES; INTEGRIN BETA-1; CYTOKINE; PROLIFERATION; IMPLANTATION; PATHOGENESIS; FREQUENCY; TSPAN5; CELLS; WOMEN;

D O I：

10.1093/femspd/ftad018

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Chlamydia trachomatis predisposes to tubal pregnancy by dysregulating tspans, activin-A and inhibin-A. Mechanism of Chlamydia trachomatis causing tubal ectopic pregnancy (EP) is not well understood. Tetraspanins (tspans), activin-A, and inhibin-A might play a role in the development of pathological conditions leading to EP. The study aimed to elucidate the expression of tspans, activin-A, and inhibin-A with a role of associated cytokines in C. trachomatis-associated EP and analyze interacting partners of DEGs, with an expression of a few important interacting genes. Fallopian tissue and serum were collected from 100 EP (Group I) and 100 controls (Group II) from SJH, New Delhi, India. Detection of C. trachomatis was done by polymerase chain reaction (PCR) and IgG antibodies were detected by enzyme-linked immunosorbent assay. Expression of tspans, activin-A, inhibin-A, and cytokines was analyzed by real time (RT)-PCR and their interacting genes were assessed by STRING. Expression of few disease-associated interacting genes was studied by RT-PCR. A total of 29% (Group I) were C. trachomatis positive. Tspans and activin-A were significantly upregulated, while inhibin-A was significantly downregulated in Group Ia. ITGA1, TLR-2, ITGB2, and Smad-3 were a few interacting genes. Expression of ITGA1, TLR-2, and Smad-3 was significantly upregulated in C. trachomatis-positive EP. Results suggested dysregulated tspans, activin-A, and inhibin-A might play a role in C. trachomatis-infected tubal EP.

引用

页数：10

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