Comprehensive metabolic profiling of Geotrichum candidum and comparison with Saccharomyces cerevisiae

被引：4

作者：

Bamba, Takahiro ^{[1
]}

Hori, Yoshimi ^{[1
]}

Umebayashi, Kyohei ^{[1
]}

Soh, Chieko ^{[2
]}

Hakozaki, Tomohiro ^{[3
]}

Toyama, Kazumi ^{[2
]}

Osumi, Masako ^{[4
]}

Kondo, Akihiko ^{[1
,5
,6
]}

Hasunuma, Tomohisa ^{[1
,5
,6
]}

机构：

[1] Kobe Univ, Engn Biol Res Ctr, 1-1 Rokkodai, Nada, Kobe 6578501, Japan

[2] Procter & Gamble Innovat GK, 7-1-18 Onoedori,Chuo Ku, Kobe, Hyogo 6510088, Japan

[3] Procter & Gamble Co, 1 P&G Plaza, Cincinnati, OH 45202 USA

[4] Japan Womens Univ, 2-8-1 Mejirodai,Bunkyo Ku, Tokyo 1128681, Japan

[5] Kobe Univ, Grad Sch Sci Technol & Innovat, 1-1 Rokkodai, Nada, Kobe 6578501, Japan

[6] RIKEN Ctr Sustainable Resource Sci, 1-7-22 Suehiro Cho,Tsurumi Ku, Yokohama, Kanagawa 2300045, Japan

来源：

JOURNAL OF BIOSCIENCE AND BIOENGINEERING | 2024年 / 137卷 / 01期

关键词：

Saccharomyces cerevisiae; Geotrichum candidum; Ultrastructure; Metabolome analysis; Pantothenic acid; GLUTATHIONE; GROWTH; BIOMARKER;

D O I：

10.1016/j.jbiosc.2023.10.004

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Geotrichum candidum is a dimorphic yeast used in cheese processing. To our knowledge, no major metabolites have been identified to date in G. candidum except for some amino acid and fatty acid metabolites. This has limited research on the commercial use of G. candidum. In this study, we aimed to analyze temporal changes in the intra- and extra-cellular metabolites of G. candidum and Saccharomyces cerevisiae cultured in YM medium as reference. As a result of metabolite analysis, it was observed that G. candidum tends to accumulate pentose phosphate pathway compounds, which are involved in nucleic acid synthesis, after 48 h of cultivation when compared to S. cerevisiae. In addition, G. candidum accumulated higher amounts of the antioxidant glutathione in the medium than did S. cerevisiae. In addition, G. candidum accumulated large amounts of B vitamins such as pantothenic acid and nicotinic acid in the medium. Finally, we examined the potential of G. candidum as a host for the production of useful compounds such as pantothenic acid. When cultured in medium supplemented with the pantothenic acid precursor b-alanine, G. candidum produced 12-fold higher amounts of pantothenic acid (30 mM) than that by S. cerevisiae. This study indicates that G. candidum accumulates various useful compounds that are dissimilar to those produced by S. cerevisiae. Furthermore, G. candidum has the potential to produce useful chemicals under appropriate culture conditions.(c) 2023, The Society for Biotechnology, Japan. All rights reserved.

引用

页码：9 / 15

页数：7

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