Aberrant DNA methylation of CDKN2B gene in the Type 1 diabetes mellitus and analysis of epigenetic markers

被引：0
作者：
Vijayaragavan, P. [1 ]
Purushothaman, Sumitha [1 ]
Arokiyaraj, Selvaraj [2 ]
Rathi, M. A. [3 ,4 ]
Gopalakrishnan, V. K. [5 ,8 ]
Gatasheh, Mansour K. [6 ]
Sivanesan, Ravikumar [7 ]
机构：
[1] Smykon Biotech Pvt LtD, Bioproc Engn Div, Kanyakumari 629201, Tamilnadu, India
[2] Sejong Univ, Dept Food Sci & Biotechnol, Seoul 05006, South Korea
[3] Karpagam Acad Higher Educ, Dept Biochem, FASCM, Coimbatore 641021, Tamil Nadu, India
[4] Karpagam Acad Higher Educ, Canc Res Ctr, FASCM, Coimbatore 641021, Tamil Nadu, India
[5] Bule Hora Univ, Inst Hlth, Sch Med, BuleHora, Ethiopia
[6] King Saud Univ, Coll Sci, Dept Biochem, POB 2455, Riyadh 11451, Saudi Arabia
[7] Rajesh Serfoji Govt Coll Autonomous, Dept Zool, Thanjavur 613005, India
[8] Karpagam Acad Higher Educ, Ctr Plant Tissue Culture & Cent Instrumentat Facil, Dept Biochem, Coimbatore 641021, Tamil Nadu, India
来源：
JOURNAL OF KING SAUD UNIVERSITY SCIENCE | 2023年 / 35卷 / 08期
关键词：
DNA methylation; Aberrant; Expression; Diabetes mellitus; Prognostic markers; P15 PROMOTER METHYLATION; NONCODING RNA; CELL-CYCLE; CANCER; DISEASE;
D O I：
10.1016/j.jksus.2023.102896
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Introduction: Aberrant DNA methylation is one of the important molecular markers in acute lymphoblastic leukemia (ALL).Objectives: To determine Aberrant DNA methylation profiles of the CDKN2A and CDKN2B genes in ALL samples from T1DM cases.Results: Aberrant hypermethylation of CDKN2B gene and cytogenetic abnormalities were analyzed in >60% of subjected cases. Total cholesterol, triglycerides and low-density lipoprotein cholesterol were not significantly varied between experimental and control (p >0.05). Expression analysis of human peripheral blood mononuclear cells revealed decreased mRNA levels of CDKN2B. The decreased level of p21 and CDK4 expression in the T1DM cases than control. The number of monocytes, lymphocytes and neutrophils significantly varied in T1DM samples and control (p < 0.05).Conclusions: Aberrant methylation is one of the important mechanisms involved in the expression of CDKN2B genes and is an important prognostic marker for the early detection of ALL risks.(c) 2023 The Author(s). Published by Elsevier B.V. on behalf of King Saud University. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
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