An efficient and sustainable furfurylamine production from biomass-derived furfural by a robust mutant co-transaminase biocatalyst

Furfurylamine is a key furan-based compound for manufacturing perfumes, fibers, additives, medicines and agrochemicals. It can be obtained by amination of furfural by co-transaminase (AtAT) from Aspergillus terreus. In this work, site-directed mutant of amino acid residues [Threonine (T) at AT130 was mutated to Methionine (M) and Glutamic acid (E) at AT133 was mutated to Phenylalanine (F)] was used to change in the flexible region of AtAT. The transamination activity and thermostability were significantly improved. In ChCl:MA (30 wt%), furfural (500 mM) was efficiently transformed into furfurylamine (92% yield) with TMEF after 12 h. 101.3 mM of biomass-derived furfural and 129.7 mM of D-xylose-derived furfural were wholly converted into furfurylamine within 5 h, achieving the productivity of 0.465 g furfurylamine/(g xylan in corncob) and 0.302 g furfurylamine/ (g D-xylose). This established chemoenzymatic conversion strategy by bridging chemocatalysis and biocatalysis could be utilized in the valorisation of renewable biomass to valuable furans.