Anti-EMT properties of ergothioneine attenuate lipopolysaccharide-induced oxidative stress-mediated acute lung injury via modulating TGF-β/smad/snail signaling pathway

被引:4
作者
Iqbal, Shabnoor [1 ]
Jabeen, Farhat [1 ]
Aslam, Noman [2 ]
Manan, Maria [3 ]
机构
[1] Govt Coll Univ, Fac Life Sci, Dept Zool, Faisalabad 38040, Pakistan
[2] Huazhong Agr Univ, Coll Food Sci & Technol, Wuhan, Peoples R China
[3] Govt Coll Univ, Fac Pharmaceut Sci, Dept Pharmacol, Faisalabad, Pakistan
关键词
NF-Kb; acute lung injury; snail; vimentin; e-cadherin; oxidative stress; inflammation; NF-KAPPA-B; EPITHELIAL-MESENCHYMAL TRANSITION; XANTHINE-OXIDASE; ASCORBIC-ACID; AMINO-ACID; ANTIOXIDANT; INFLAMMATION; MECHANISMS; MODEL; SNAIL;
D O I
10.1177/09603271231178015
中图分类号
R99 [毒物学(毒理学)];
学科分类号
100405 ;
摘要
Acute lung injury (ALI) is a heterogeneous pulmonary illness that is fast developing and has a high fatality rate. The current investigation set out to interpret the convergence of oxidative stress, inflammatory cytokines, TNF-alpha, snail, vimentin, e-cadherin, and NF-kB activation in ALI pathology. The outcome of assays of oxidative stress, ELISA, and western blot showed the declined of CAT, SOD, GPx, IL-1 beta, TNF-alpha, and upregulation of TGF-beta, smad2/3, smad4, NF-kB, snail, and vimentin, concurrently with downregulation of e-cadherin expression in lung tissues as well as BALF in LPS-injected rats. The photomicrographs of the lungs marked severe congestion, infiltration of cytokines, and thickening of the alveolar walls. Pretreatments of ergothioneine after LPS-induced ALI, inhibited EMT-induction by blocking TGF-beta, smad2/3, smad4, snail, vimentin, NF-kB, and inflammatory cytokines, and increased the expression of E-cadherin and antioxidant levels in a dose-dependent manner. These events helped to restore lung histoarchitecture and reduce acute lung injury. The present findings suggest that ergothioneine at 100 mg/kg is as effective as febuxostat (reference drug). The study concluded that ergothioneine may be replaced with febuxostat as a treatment option for ALI owing to its side effects after clinical trials for pharmaceutical purposes.
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页数:11
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