Phenotypic profiling of CD34+ cells by advanced flow cytometry improves diagnosis of juvenile myelomonocytic leukemia

被引:0
作者
Bugarin, Cristina [1 ]
Antolini, Laura [2 ]
Buracchi, Chiara [1 ,2 ]
Matarraz, Sergio [3 ,4 ]
Coliva, Tiziana Angela [5 ]
van der Velden, Vincent H. [6 ]
Szczepanski, Tomasz [7 ]
da Costa, Elaine Sobral [8 ]
van der Sluijs, Alita [9 ]
Novakova, Michaela [10 ,11 ]
Mejstrikova, Ester [10 ,11 ]
Nierkens, Stefan [12 ]
de Mello, Fabiana Vieira [8 ]
Fernandez, Paula [13 ]
Aanei, Carmen [2 ,14 ]
Sedek, Lukasz [7 ]
Strocchio, Luisa [15 ]
Masetti, Riccardo [16 ]
Sainati, Laura [17 ]
Philippe, Jan [18 ]
Valsecchi, Maria Grazia [2 ]
Locatelli, Franco [15 ]
van Dongen, Jacques J. M. [3 ,4 ,9 ]
Biondi, Andrea [1 ,19 ]
Orfao, Alberto [3 ,4 ]
Gaipa, Giuseppe [1 ]
机构
[1] Fdn IRCCS San Gerardo Tintori, Ctr Tettamanti, Monza, MB, Italy
[2] Univ Milano Bicocca, Ctr Biostat Clin Epidemiol, Dipartimento Med & Chirurg, Monza, MB, Italy
[3] Univ Salamanca, CSIC, Dept Med & Cytometry Serv NUCLEUS, CIBERONC,Canc Res Ctr IBMCC, Salamanca, Spain
[4] Inst Biomed Res Salamanca IBSAL, Salamanca, Spain
[5] Fdn IRCCS San Gerardo Tintori, Dept Pediat, Monza, MB, Italy
[6] Univ Med Ctr Rotterdam, Dept Immunol, Erasmus MC, Rotterdam, Netherlands
[7] Med Univ Silesia SUM, Dept Pediat Hematol & Oncol, Zabrze, Poland
[8] Univ Fed Rio de Janeiro, Dept Pediat, Rio De Janeiro, Brazil
[9] Leiden Univ Med Ctr LUMC, Dept Immunohematol & Blood Transfus IHB, Leiden, Netherlands
[10] Charles Univ Prague, Fac Med 2, Dept Pediat Hematol & Oncol, CLIP, Prague, Czech Republic
[11] Univ Hosp Motol, Prague, Czech Republic
[12] Princess Maxima Ctr Pediat Oncol, Utrecht, Netherlands
[13] Kantonsspital Aarau AG, Inst Lab Med, Aarau, Switzerland
[14] CHU St Etienne, Hematol Lab, St Etienne, France
[15] Sapienza Univ Rome, IRCCS Osped Pediat Bambino Gesu, Dept Pediat Hematol & Oncol, Rome, Italy
[16] IRCCS Osped Univ Bologna, IRCCS Ospedaliero Universitaria Bologna, Bologna, Italy
[17] Azienda Osped Univ Padova, Dipartimento Salute Donna & Bambino, Clin Oncoematol Pediat, Padua, Italy
[18] Ghent Univ Hosp, Dept Lab Med, Ghent, Belgium
[19] Univ Milano Bicocca, Dipartimento Med & Chirurg, Monza, MB, Italy
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摘要
Diagnostic criteria for juvenile myelomonocytic leukemia (JMML) are currently well defined, however in some patients diagnosis still remains a challenge. Flow cytometry is a well established tool for diagnosis and follow-up of hematological malignancies, nevertheless it is not routinely used for JMML diagnosis. Herewith, we characterized the CD34+ hematopoietic precursor cells collected from 31 children with JMML using a combination of standardized EuroFlow antibody panels to assess the ability to discriminate JMML cells from normal/reactive bone marrow cell as controls (n=29) or from cells of children with other hematological diseases mimicking JMML (n=9). CD34+ precursors in JMML showed markedly reduced B -cell and erythroid-committed precursors compared to controls, whereas monocytic and CD7+ lymphoid precursors were significantly expanded. Moreover, aberrant immunophenotypes were consistently present in CD34+ precursors in JMML, while they were virtually absent in controls. Multivariate logistic regression analysis showed that combined assessment of the number of CD34+CD7+ lymphoid precursors and CD34+ aberrant precursors or erythroid precursors had a great potential in discriminating JMMLs versus controls. Importantly our scoring model allowed highly efficient discrimination of truly JMML versus patients with JMML-like diseases. In conclusion, we show for the first time that CD34+ precursors from JMML patients display a unique immunophenotypic profile which might contribute to a fast and accurate diagnosis of JMML worldwide by applying an easy to standardize single eight -color antibody combination.
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页码:521 / 532
页数:12
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