Exploring the Immunomodulatory Properties of Red Onion (Allium cepa L.) Skin: Isolation, Structural Elucidation, and Bioactivity Study of Novel Onion Chalcones Targeting the A2A Adenosine Receptor

被引：1

作者：

Zhang, Rui-Jia ^{[1
,2
,3
]}

Rao, Qian-Ru ^{[4
]}

Jiang, Xue-Qin ^{[2
,3
]}

Ye, Neng ^{[2
,3
]}

Li, Na ^{[2
,3
]}

Du, Hong-Ling ^{[2
,3
]}

Zhang, Shun-Jie ^{[2
,3
]}

Ye, Hao-Yu ^{[2
,3
]}

Wu, Wen-Shuang ^{[5
,6
]}

Zhao, Min ^{[1
]}

机构：

[1] Sichuan Univ, West China Hosp, Dept Urol, Inst Urol,Lab Reconstruct Urol, Chengdu 610041, Sichuan, Peoples R China

[2] Sichuan Univ, West China Hosp, Canc Ctr, Dept Biotherapy, Chengdu 610041, Sichuan, Peoples R China

[3] Sichuan Univ, West China Hosp, State Key Lab Biotherapy, Chengdu 610041, Sichuan, Peoples R China

[4] Sichuan Univ, West China Univ Hosp 2, Minist Educ, Lab Mol Translat Med,Ctr Translat Med,Key Lab Birt, Chengdu 610041, Sichuan, Peoples R China

[5] Sichuan Univ, West China Hosp, Frontiers Sci Ctr Dis Related Mol Network, Div Thyroid Surg,Dept Gen Surg, Chengdu 610041, Sichuan, Peoples R China

[6] Sichuan Univ, West China Hosp, Frontiers Sci Ctr Dis Related Mol Network, Lab Thyroid & Parathyroid Dis, Chengdu 610041, Sichuan, Peoples R China

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2023年 / 71卷 / 45期

基金：

中国国家自然科学基金;

关键词：

Allium cepa L; chalcone; A(2A) adenosine receptor; T-cell activation; immunomodulatory property; ANTIOXIDANT; CONSTITUENTS; DERIVATIVES; ENZYME; ROOTS;

D O I：

10.1021/acs.jafc.3c03851

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

Onions are versatile and nutritious food widely used in various cuisines around the world. In our ongoing pursuit of bioactive substances with health benefits from red onion (Allium cepa L.) skin, a comprehensive chemical investigation was undertaken. Consequently, a total of 44 compounds, including three previously unidentified chalcones (1-3) were extracted from red onion skin. Of these isolates, chalcones 1-4 showed high affinity to A(2A) adenosine receptor (A(2A)AR), and chalcone 2 displayed the best binding affinity to A(2A)AR, with the IC50 value of 33.5 nM, good A(2A)AR selectivity against A(1)AR, A(2B)AR, and A(3)AR, and high potency in the cAMP functional assay (IC50 of 913.9 nM). Importantly, the IL-2 bioassay and the cell-mediated cytotoxicity assay demonstrated that chalcone 2 could boost T-cell activation. Furthermore, the binding mechanism of chalcone 2 with hA(2A)AR was elucidated by molecular docking. This work highlighted that the active chalcones in red onion might have the potential to be developed as A(2A)AR antagonists used in cancer immunotherapy.

引用

页码：17046 / 17055

页数：10