The main application possibilities of the LAMP (Loop-mediated Isothermal Amplification) technique in veterinary science

被引:1
作者
Zorkoczy, K. Orsolya [1 ]
Bujtor, Zsofia [1 ]
Szives, Andras [1 ]
Ozsvari, Laszlo [2 ,3 ]
Wagenhoffer, Zsornbor [1 ]
Zenke, Petra [1 ]
机构
[1] Allatorvostudomanyi Egyet, Allattenyesztesi Takarmanyozastani Lab, Istvan Uutca 2, H-1078 Budapest, Hungary
[2] Allatorvostudomonyi Egyet, Gazdasagtudomanyi Stat Int, Torvenyszeki Ailarorvosrani GazdasagtudomOnyi Tan, Budapest, Hungary
[3] Allatorvostudomanyi Egyet, Fertozo Allatheregsegek Antimikrobialis Reziszte, Allatorvcsi Kazegesz Segugy Elelmiszerlancbizton, Budapest, Hungary
关键词
ON-SITE DETECTION; RAPID DETECTION; SENSITIVE DETECTION; MOLECULAR-DETECTION; DNA AMPLIFICATION; SEX DETERMINATION; VISUAL DETECTION; GENOMIC DNA; ASSAY; VIRUS;
D O I
10.56385/magyallorv.2023.10.611-624
中图分类号
S85 [动物医学(兽医学)];
学科分类号
0906 ;
摘要
The Loop-mediated Isothermal Amplification (LAMP) technique has various application possibilities in veterinary sciences, particularly in diagnosing infectious diseases, animal husbandry, and food testing. In this summary, the authors show how the technique is utilized in these fields. LAMP is widely used in veterinary medicine for the sensitive, rapid and accurate diagnosis of infectious diseases in animals. The technique allows for the detection of specific DNA or RNA sequences of pathogens present in clinical samples, such as blood, tissues, urine, a feces, or swabs. LAMP is highly sensitive and specific, and can provide results as quickly as 30-60 minutes, making it valuable in identifying pathogens responsible for diseases in livestock and pets. It aids in early detection, surveillance, and control of infectious diseases, allowing for prompt treatment and prevention measures. LAMP can also be applied to determine the sex of several domestic animals. This information allows for the selection of specific traits associated with either sex and optimizing breeding strategies, and it is crucial for dairy and beef cattle breeding programs. To ensure the safety and quality of animal-derived food products, LAMP can be used to identify the species components of meat and milk products, and therefore, can detect adulteration or mislabeling. This helps to ensure the accuracy of labeling, meet regulatory requirements, and prevent fraudulent practices in the meat and dairy industry. Overall, LAMP's simplicity, rapidity and accuracy make it a promising cost-efficient diagnostic tool with the potential for on-site applications of a large number of samples in veterinary practice.
引用
收藏
页码:611 / 624
页数:14
相关论文
共 50 条
  • [41] Loop-Mediated Isothermal Amplification (LAMP): Emergence As an Alternative Technology for Herbal Medicine Identification
    Li, Jing-jian
    Xiong, Chao
    Liu, Yue
    Liang, Jun-song
    Zhou, Xing-wen
    FRONTIERS IN PLANT SCIENCE, 2016, 7
  • [42] The application of loop-mediated isothermal amplification (LAMP) in food testing for bacterial pathogens and fungal contaminants
    Niessen, Ludwig
    Luo, Jie
    Denschlag, Carla
    Vogel, Rudi F.
    FOOD MICROBIOLOGY, 2013, 36 (02) : 191 - 206
  • [43] Application of a Loop-Mediated Isothermal Amplification (LAMP) Assay for the Detection of Listeria monocytogenes in Cooked Ham
    Fiore, Alfonsina
    Treglia, Ida
    Ciccaglioni, Gianni
    Ortoffi, Marco Francesco
    Gattuso, Antonietta
    FOODS, 2023, 12 (01)
  • [44] Recent advances in loop-mediated isothermal amplification (LAMP) for rapid and efficient detection of pathogens
    Garg, Nupur
    Ahmad, Farhan Jalees
    Kar, Sudeshna
    CURRENT RESEARCH IN MICROBIAL SCIENCES, 2022, 3
  • [45] Development and standardization of a Loop-mediated isothermal amplification (LAMP) test for the detection of Babesia bigemina
    Lizarazo-Zuluaga, Andrea P.
    Carvajal-Gamez, Bertha I.
    Wilkowsky, Silvina
    Cravero, Silvio
    Trangoni, Marcos
    Mosqueda, Juan
    FRONTIERS IN VETERINARY SCIENCE, 2022, 9
  • [46] Development of Loop-Mediated Isothermal Amplification (LAMP) Assays for Rapid Detection of Ehrlichia ruminantium
    Nakao, Ryo
    Stromdahl, Ellen Y.
    Magona, Joseph W.
    Faburay, Bonto
    Namangala, Boniface
    Malele, Imna
    Inoue, Noboru
    Geysen, Dirk
    Kajino, Kiichi
    Jongejan, Frans
    Sugimoto, Chihiro
    BMC MICROBIOLOGY, 2010, 10
  • [47] Pullulan reduces the non-specific amplification of loop-mediated isothermal amplification (LAMP)
    Gao, Xueqin
    Sun, Bingqi
    Guan, Yifu
    ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 2019, 411 (06) : 1211 - 1218
  • [48] A rapid detection method for the plant pathogen Sclerotinia sclerotiorum based on loop-mediated isothermal amplification (LAMP)
    Duan, Yabing
    Ge, Changyan
    Zhang, Xiaoke
    Wang, Jianxin
    Zhou, Mingguo
    AUSTRALASIAN PLANT PATHOLOGY, 2014, 43 (01) : 61 - 66
  • [49] Detection of chronic toxoplasmosis in the brain of mice using loop-mediated isothermal amplification (LAMP) and conventional PCR
    Hegazy, Mona K.
    Saleh, Nora E.
    Aboukamar, Wafaa A.
    EXPERIMENTAL PARASITOLOGY, 2023, 251
  • [50] Loop-mediated isothermal amplification (LAMP) approach for detection of heat-resistant Talaromyces flavus species
    Panek, Jacek
    Frac, Magdalena
    SCIENTIFIC REPORTS, 2019, 9 (1)