The main application possibilities of the LAMP (Loop-mediated Isothermal Amplification) technique in veterinary science

被引:1
作者
Zorkoczy, K. Orsolya [1 ]
Bujtor, Zsofia [1 ]
Szives, Andras [1 ]
Ozsvari, Laszlo [2 ,3 ]
Wagenhoffer, Zsornbor [1 ]
Zenke, Petra [1 ]
机构
[1] Allatorvostudomanyi Egyet, Allattenyesztesi Takarmanyozastani Lab, Istvan Uutca 2, H-1078 Budapest, Hungary
[2] Allatorvostudomonyi Egyet, Gazdasagtudomanyi Stat Int, Torvenyszeki Ailarorvosrani GazdasagtudomOnyi Tan, Budapest, Hungary
[3] Allatorvostudomanyi Egyet, Fertozo Allatheregsegek Antimikrobialis Reziszte, Allatorvcsi Kazegesz Segugy Elelmiszerlancbizton, Budapest, Hungary
关键词
ON-SITE DETECTION; RAPID DETECTION; SENSITIVE DETECTION; MOLECULAR-DETECTION; DNA AMPLIFICATION; SEX DETERMINATION; VISUAL DETECTION; GENOMIC DNA; ASSAY; VIRUS;
D O I
10.56385/magyallorv.2023.10.611-624
中图分类号
S85 [动物医学(兽医学)];
学科分类号
0906 ;
摘要
The Loop-mediated Isothermal Amplification (LAMP) technique has various application possibilities in veterinary sciences, particularly in diagnosing infectious diseases, animal husbandry, and food testing. In this summary, the authors show how the technique is utilized in these fields. LAMP is widely used in veterinary medicine for the sensitive, rapid and accurate diagnosis of infectious diseases in animals. The technique allows for the detection of specific DNA or RNA sequences of pathogens present in clinical samples, such as blood, tissues, urine, a feces, or swabs. LAMP is highly sensitive and specific, and can provide results as quickly as 30-60 minutes, making it valuable in identifying pathogens responsible for diseases in livestock and pets. It aids in early detection, surveillance, and control of infectious diseases, allowing for prompt treatment and prevention measures. LAMP can also be applied to determine the sex of several domestic animals. This information allows for the selection of specific traits associated with either sex and optimizing breeding strategies, and it is crucial for dairy and beef cattle breeding programs. To ensure the safety and quality of animal-derived food products, LAMP can be used to identify the species components of meat and milk products, and therefore, can detect adulteration or mislabeling. This helps to ensure the accuracy of labeling, meet regulatory requirements, and prevent fraudulent practices in the meat and dairy industry. Overall, LAMP's simplicity, rapidity and accuracy make it a promising cost-efficient diagnostic tool with the potential for on-site applications of a large number of samples in veterinary practice.
引用
收藏
页码:611 / 624
页数:14
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