In situ SERS imaging of protein-specific glycan oxidation on living cells to quantitatively visualize pathogen-cell interactions

被引：3

作者：

Wang, Yuru ^{[1
]}

Wu, Shan ^{[1
]}

Yang, Yuanjiao ^{[1
]}

Yang, Yuhui ^{[1
]}

Liu, Huipu ^{[1
]}

Chen, Yunlong ^{[1
]}

Ju, Huangxian ^{[1
]}

机构：

[1] Nanjing Univ, Sch Chem & Chem Engn, State Key Lab Analyt Chem Life Sci, Nanjing 210023, Peoples R China

来源：

CHEMICAL SCIENCE | 2024年 / 15卷 / 11期

基金：

中国国家自然科学基金;

关键词：

HYDROGEN-PEROXIDE; FUSARIUM; NANOPARTICLES; MOLECULES; CHEMISTRY;

D O I：

10.1039/d4sc00157e

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Glycan oxidation on the cell surface occurs in many specific life processes including pathogen-cell interactions. This work develops a surface-enhanced Raman scattering (SERS) imaging strategy for in situ quantitative monitoring of protein-specific glycan oxidation mediated pathogen-cell interactions by utilizing Raman reporter DTNB and aptamer co-assembled platinum shelled gold nanoparticles (Au@Pt-DTNB/Apt). Using Fusarium graminearum (FG) and MCF-7 cells as models, Au@Pt-DTNB/Apt can specifically bind to MUC1 protein on the cell surface containing heavy galactose (Gal) and N-acetylgalactosamine (GalNAc) modification. When FG interacts with cells, the secreted galactose oxidase (GO) can oxidize Gal/GalNAc, and the generated reactive oxygen species (ROS) further oxidizes DTNB to produce TNB for greatly enhancing the SERS signal. This strategy can quantitatively visualize for the first time both the protein-specific glycan oxidation and the mediated pathogen-cell interactions, thus providing key quantitative information to distinguish and explore the pathogen-resistance and pharmacological mechanisms of different drugs.

引用

页码：3901 / 3906

页数：6

共 37 条

[1] CARBOHYDRATE-SPECIFIC RECEPTORS OF THE LIVER
ASHWELL, G
HARFORD, J
[J]. ANNUAL REVIEW OF BIOCHEMISTRY, 1982, 51 : 531 - 554
[2] Glycosylation and rheumatic disease
Axford, JS
[J]. BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE, 1999, 1455 (2-3): : 219 - 229
[3] Review of iron-free Fenton-like systems for activating H2O2 in advanced oxidation processes
Bokare, Alok D.
Choi, Wonyong
[J]. JOURNAL OF HAZARDOUS MATERIALS, 2014, 275 : 121 - 135
[4] Fusarium, a significant emerging pathogen in patients with hematologic malignancy: Ten years' experience at a cancer center and implications for management
Boutati, EI
Anaissie, EJ
[J]. BLOOD, 1997, 90 (03) : 999 - 1008
[5] Oxidative Damage of Tryptophan Hydroxylase-2 Mediated by Peroxisomal Superoxide Anion Radical in Brains of Mouse with Depression
Ding, Qi
Tian, Ying
Wang, Xin
Li, Ping
Su, Di
Wu, Chuanchen
Zhang, Wen
Tang, Bo
[J]. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2020, 142 (49) : 20735 - 20743
[6] Sensing Glucose in Urine and Serum and Hydrogen Peroxide in Living Cells by Use of a Novel Boronate Nanoprobe Based on Surface-Enhanced Raman Spectroscopy
Gu, Xin
Wang, Hao
Schultz, Zachary D.
Camden, Jon P.
[J]. ANALYTICAL CHEMISTRY, 2016, 88 (14) : 7191 - 7197
[7] Guo YA, 2023, J AM CHEM SOC, V145, P5092, DOI 10.1021/jacs.2c11150
[8] Hattrup CL, 2008, ANNU REV PHYSIOL, V70, P431, DOI 10.1146/annurev.physiol.70.113006.100659
[9] Hong SJ, 2019, NAT COMMUN, V10, DOI [10.1038/s41467-019-08944-1, 10.1038/s41467-019-09608-w]
[10] Decreased capacity of recombinant 45/47-kDa molecules (Apa) of Mycobacterium tuberculosis to stimulate T lymphocyte responses related to changes in their mannosylation pattern
Horn, C
Namane, A
Pescher, P
Rivière, M
Romain, F
Puzo, G
Bârzu, O
Marchal, G
[J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1999, 274 (45) : 32023 - 32030

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