Mental fatigue (MF) has been shown to acutely impair the psychological responses and endurance running performance of orienteers. This study aimed to explore MF levels experienced by orienteers during a 4-day competition preparation camp that consisted of simulated sprint, middle-distance, long-distance, relay and night races. Eleven national junior orienteers participated in the study (age: 15-17 years, height: 1.69 +/- 0.07 m and body mass: 59.9 +/- 5.22 kg). Subjective ratings of MF, motivation, stress, physical fatigue (PF) and tiredness were measured using a 100-mm visual analogue scale. The Brunel Mood Scale (BRUMS) was utilized to assess the mood state of the orienteers. The self-report measures were taken within 30 min of waking, immediately after the post-training session, and after 24 and 48 h following the final training session. The pre-post orienteering training combined analysis showed that there was a moderate increase in perceived MF (ES = 1.06 [0.66, 1.45]), PF (ES = 1.07 [0.69, 1.45]) and BRUMS fatigue (ES = 0.74 [0.4, 1.1]) after orienteering training. At 48 h post the final training session, MF remained moderately elevated (ES = 0.86 [-0.07, 1.75]), while PF also remained elevated to a small extent (ES = 0.46 [-0.46, 1.39]) compared to the pre-training values. A moderate impairment was still observed in BRUMS vigor (ES = -1.02 [-1.65, -0.36]), but BRUMS confusion scores were moderately lower (ES = -0.85 [-1.71, 0.04]) than pre-training values. This study found that orienteering training induced acute MF, persisting for at least 48 h after the final session.