Phylogenomics analysis of multidrug-resistant Elizabethkingia anophelis in industrial wastewater treatment plant

被引：1
作者：
Nascimento, Ana Paula Alves [1 ]
de Farias, Beatriz Oliveira [1 ,2 ]
Goncalves-Brito, Andressa Silva [1 ,2 ]
Magaldi, Mariana [1 ,2 ]
Flores, Claudia [1 ]
Quidorne, Camila Silva [1 ]
Montenegro, Kaylanne S. [1 ]
Bianco, Kayo [1 ,2 ,3 ]
Clementino, Maysa Mandetta [1 ,2 ,3 ]
机构：
[1] Fiocruz MS, Oswaldo Cruz Fdn, INCQS, Natl Inst Qual Control Hlth, 4365, Rio de Janeiro, RJ, Brazil
[2] Fiocruz MS, Oswaldo Cruz Fdn, Fiocruz Genom Network, 4365, Rio de Janeiro, RJ, Brazil
[3] COVID 19 Monitoring Network Wastewater, BR-09210580 Santo Andre, SP, Brazil
来源：
JOURNAL OF APPLIED MICROBIOLOGY | 2023年 / 134卷 / 09期
关键词：
Elizabethkingia anophelis; phylogenetic evolution; whole genome sequencing; wastewater; 16S RIBOSOMAL-RNA; ANTIMICROBIAL SUSCEPTIBILITY; ANTIBIOTIC-RESISTANCE; HORIZONTAL TRANSFER; MENINGITIS; OUTBREAK; BACTERIAL; PROFILES; GENES;
D O I：
10.1093/jambio/lxad215
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Aims This study investigated the phylogenetic relatedness of multidrug-resistant Elizabethkingia anophelis recovered from an industrial wastewater treatment plant (WWTPi).Methods and results The wastewater samples were plated in brain heart infusion agar (4 mg/L ceftazidime, 8 mg/L meropenem, and 2 mg/L polimixin). Four isolates recovered from four stages of WWTPi (influent, aeration, decantation, and treated effluent) were identified and evaluated of susceptibility profiles in the VITEK 2 system. These strains identified as E. meningoseptica were confirmed to be E. anophelis by whole genomic sequencing (Miseq-Illumina) and showed antimicrobial resistance genes of beta-lactams, aminoglycosides, and tetracycline's classes. The ribosomal multilocus sequence typing showed that they belong to the rST 65620 together with clinical strains. The phylogenomic tree revealed the similarity of our strains to those belonging to sublineage 11 and the single nucleotide polymorphism analysis confirmed that they belong to a single clade.Conclusions To the best of our knowledge, this is the first study reporting the persistence of multidrug-resistant E. anophelis sublineage 11 along the wastewater treatment.
引用
收藏
页数：11
相关论文
共 74 条
  • [1] METAVIRALSPADES: assembly of viruses from metagenomic data
    Antipov, Dmitry
    Raiko, Mikhail
    Lapidus, Alla
    Pevzner, Pavel A.
    [J]. BIOINFORMATICS, 2020, 36 (14) : 4126 - 4129
  • [2] Neonatal Meningitis and Septicemia Caused by Multidrug-Resistant Elizabethkingia anophelis Identified by 16s Ribosomal RNA: An Emerging Threat
    Baruah, Frincy K.
    Borkakoty, Biswajyoti
    Ahmed, Abid
    Bora, Prakritish
    [J]. JOURNAL OF GLOBAL INFECTIOUS DISEASES, 2020, 12 (04) : 225 - 227
  • [3] Benson M D., 2014, Reference Module in Biomedical Sciences, P1, DOI [10.1016/b978-0-12-801238-3.04922-9, DOI 10.1016/B978-0-12-801238-3.04922-9, 10.1016/B978-012-801238-3.05551-3]
  • [4] Genomic epidemiology and global diversity of the emerging bacterial pathogen Elizabethkingia anophelis
    Breurec, Sebastien
    Criscuolo, Alexis
    Diancourt, Laure
    Rendueles, Olaya
    Vandenbogaert, Mathias
    Passet, Virginie
    Caro, Valerie
    Rocha, Eduardo P. C.
    Touchon, Marie
    Brisse, Sylvain
    [J]. SCIENTIFIC REPORTS, 2016, 6
  • [5] Cameron A, 2018, MSPHERE, V3, DOI [10.1128/mSphere.00568-17, 10.1128/msphere.00568-17]
  • [6] In Silico Detection and Typing of Plasmids using PlasmidFinder and Plasmid Multilocus Sequence Typing
    Carattoli, Alessandra
    Zankari, Ea
    Garcia-Fernandez, Aurora
    Larsen, Mette Voldby
    Lund, Ole
    Villa, Laura
    Aarestrup, Frank Moller
    Hasman, Henrik
    [J]. ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2014, 58 (07) : 3895 - 3903
  • [7] Elizabethkingia anophelis: Clinical Experience of an Academic Health System in Southeastern Wisconsin
    Castro, Carlos E. Figueroa
    Johnson, Carrie
    Williams, Mary
    VanDerSlik, April
    Graham, Mary Beth
    Letzer, David
    Ledeboer, Nathan
    Buchan, Blake W.
    Block, Timothy
    Borlaug, Gwen
    Munoz-Price, L. Silvia
    [J]. OPEN FORUM INFECTIOUS DISEASES, 2017, 4 (04):
  • [8] MBLs, Rather Than Efflux Pumps, Led to Carbapenem Resistance in Fosfomycin and Aztreonam/Avibactam Resistant Elizabethkingia anophelis
    Chang, Yanbin
    Zhang, Daiqin
    Niu, Siqiang
    Chen, Qian
    Lin, Qiuxia
    Zhang, Xiaobing
    [J]. INFECTION AND DRUG RESISTANCE, 2021, 14 : 315 - 327
  • [9] fastp: an ultra-fast all-in-one FASTQ preprocessor
    Chen, Shifu
    Zhou, Yanqing
    Chen, Yaru
    Gu, Jia
    [J]. BIOINFORMATICS, 2018, 34 (17) : 884 - 890
  • [10] Sporadic Dissemination of tet(X3) and tet(X6) Mediated by Highly Diverse Plasmidomes among Livestock-Associated Acinetobacter
    Cheng, Ying-Ying
    Liu, Yang
    Chen, Yong
    Huang, Fu-Man
    Chen, Rong-Chang
    Xiao, Yong-Hong
    Zhou, Kai
    [J]. MICROBIOLOGY SPECTRUM, 2021, 9 (03):
12345678