Administration of Tamoxifen Can Regulate Changes in Gene Expression during the Acute Phase of Traumatic Spinal Cord Injury

被引：5

作者：

Cabrera-Aldana, Eibar E. ^{[1
,9
]}

Balderas-Martinez, Yalbi I. ^{[2
]}

Velazquez-Cruz, Rafael ^{[3
]}

Tovar-y-Romo, Luis B. ^{[4
]}

Sevilla-Montoya, Rosalba ^{[5
]}

Martinez-Cruz, Angelina ^{[6
]}

Martinez-Cordero, Claudia ^{[7
]}

Valdes-Flores, Margarita ^{[1
]}

Santamaria-Olmedo, Monica ^{[1
]}

Hidalgo-Bravo, Alberto ^{[1
]}

Guizar-Sahagun, Gabriel ^{[8
]}

机构：

[1] Natl Inst Rehabil INR, Dept Genom Med, Calzada Mexico Xochimilco 289, Mexico City 14389, Mexico

[2] Inst Nacl Enfermedades Resp, Lab Biol Computac, Calz Tlalpan 4502,Belisario Dominguez Secc 16, Mexico City 14080, Mexico

[3] Natl Inst Genom Med INMEGEN, Genom Bone Metab Lab, Periferico Sur 4809, Mexico City 14610, Mexico

[4] Univ Nacl Autonoma Mexico, Dept Mol Neuropathol, Inst Fisiol Celular, Ciudad Univ,Circuito Exterior S-N, Mexico City 04510, Mexico

[5] Natl Inst Perinatol, Reprod Res & Perinatal Hlth Dept, Montes Urales 800, Mexico City 11000, Mexico

[6] Proyecto Camina AC, Dept Expt Surg, 4430 Calz Tlalpan, Mexico City 14050, Mexico

[7] Reg Hosp High Specialty Bajio, Blvd Milenio 130, Leon 37660, Guanajuato, Mexico

[8] Inst Mexicano Seguro Social, Res Unit Neurol Dis, 330 Ave Cuauhtemoc, Mexico City 06720, Mexico

[9] Hosp Angeles Acoxpa, Calz Acoxpa 430, Mexico City 14380, Mexico

来源：

CURRENT ISSUES IN MOLECULAR BIOLOGY | 2023年 / 45卷 / 09期

关键词：

spinal cord injury; transcriptome; tamoxifen; gene expression; inflammatory response; IMPROVES LOCOMOTOR RECOVERY; GENDER; SERVER; DAMAGE; MOUSE; DRUG; RATS;

D O I：

10.3390/cimb45090472

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Traumatic spinal cord injury (SCI) causes irreversible damage leading to incapacity. Molecular mechanisms underlying SCI damage are not fully understood, preventing the development of novel therapies. Tamoxifen (TMX) has emerged as a promising therapy. Our aim was to identify transcriptome changes in the acute phase of SCI and the effect of Tamoxifen on those changes in a rat model of SCI. Four groups were considered: (1) Non-injured without TMX (Sham/TMX-), (2) Non-injured with TMX (Sham/TMX+), (3) injured without TMX (SCI/TMX-), and (4) injured with TMX (SCI/TMX+). Tamoxifen was administered intraperitoneally 30 min after injury, and spinal cord tissues were collected 24 h after injury. Clariom S Assays Array was used for transcriptome analysis. After comparing Sham/TMX- versus SCI/TMX-, 708 genes showed differential expression. The enriched pathways were the SCI pathway and pathways related to the inflammatory response. When comparing SCI/TMX- versus SCI/TMX+, only 30 genes showed differential expression, with no pathways enriched. Our results showed differential expression of genes related to the inflammatory response after SCI, and Tamoxifen seems to regulate gene expression changes in Ccr2 and Mmp12. Our study contributes data regarding the potential value of tamoxifen as a therapeutic resource for traumatic SCI during the acute phase.

引用

页码：7476 / 7491

页数：16

共 50 条

[1] Traumatic spinal cord injury [J].

Ahuja, Christopher S. ;

Wilson, Jefferson R. ;

Nori, Satoshi ;

Kotter, Mark R. N. ;

Druschel, Claudia ;

Curt, Armin ;

Fehlings, Michael G. .

NATURE REVIEWS DISEASE PRIMERS, 2017, 3

[2] Expression of pro-inflammatory cytokine and chemokine mRNA upon experimental spinal cord injury in mouse: An in situ hybridization study [J].

Bartholdi, D ;

Schwab, ME .

EUROPEAN JOURNAL OF NEUROSCIENCE, 1997, 9 (07) :1422-1438

[3] Graded histological and locomotor outcomes after spinal cord contusion using the NYU weight-drop device versus transection [J].

Basso, DM ;

Beattie, MS ;

Bresnahan, JC .

EXPERIMENTAL NEUROLOGY, 1996, 139 (02) :244-256

[4]

Blighe K., 2021, EnhancedVolcano: Publication-ready volcano plots with enhanced colouring and labeling, DOI DOI 10.18129/B9.BIOC.ENHANCEDVOLCANO

[5] A framework for oligonucleotide microarray preprocessing [J].

Carvalho, Benilton S. ;

Irizarry, Rafael A. .

BIOINFORMATICS, 2010, 26 (19) :2363-2367

[6] MMP-12, a Promising Therapeutic Target for Neurological Diseases [J].

Chelluboina, Bharath ;

Nalamolu, Koteswara Rao ;

Klopfenstein, Jeffrey D. ;

Pinson, David M. ;

Wang, David Z. ;

Vemuganti, Raghu ;

Veeravalli, Krishna Kumar .

MOLECULAR NEUROBIOLOGY, 2018, 55 (02) :1405-1409

[7] RNA-Seq Characterization of Spinal Cord Injury Transcriptome in Acute/Subacute Phases: A Resource for Understanding the Pathology at the Systems Level [J].

Chen, Kenian ;

Deng, Shuyun ;

Lu, Hezuo ;

Zheng, Yiyan ;

Yang, Guodong ;

Kim, Dong ;

Cao, Qilin ;

Wu, Jia Qian .

PLOS ONE, 2013, 8 (08)

[8] Changing Demographics and Injury Profile of New Traumatic Spinal, Cord Injuries in the United States, 1972-2014 [J].

Chen, Yuying ;

He, Yin ;

DeVivo, Michael J. .

ARCHIVES OF PHYSICAL MEDICINE AND REHABILITATION, 2016, 97 (10) :1610-1619

[9] Continuous tamoxifen delivery improves locomotor recovery 6 h after spinal cord injury by neuronal and glial mechanisms in male rats [J].

Colon, Jennifer M. ;

Gonzalez, Pablo A. ;

Cajigas, Ambar ;

Maldonado, Wanda I. ;

Torrado, Aranza I. ;

Sandago, Jose M. ;

Salgado, Iris K. ;

Miranda, Jorge D. .

EXPERIMENTAL NEUROLOGY, 2018, 299 :109-121

[10] Tamoxifen: an FDA approved drug with neuroprotective effects for spinal cord injury recovery [J].

Colon, Jennifer M. ;

Miranda, Jorge D. .

NEURAL REGENERATION RESEARCH, 2016, 11 (08) :1208-1211

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