LC-MS based metabolic profiling and wound healing activity of a chitosan nanoparticle-loaded formula of Teucrium polium in diabetic rats

被引:8
作者
Algandaby, Mardi M. [1 ]
Esmat, Ahmed [2 ]
Nasrullah, Mohammed Z. [3 ]
Alhakamy, Nabil A. [4 ,5 ,6 ]
Abdel-Naim, Ashraf B. [3 ]
Rashad, Omar M. [7 ]
Elhady, Sameh S. [8 ]
Eltamany, Enas E. [9 ]
机构
[1] King Abdulaziz Univ, Fac Sci, Dept Biol Sci, Jeddah 21589, Saudi Arabia
[2] King Abdulaziz Univ, Fac Med, Dept Pharmacol, Jeddah 21589, Saudi Arabia
[3] King Abdulaziz Univ, Fac Pharm, Dept Pharmacol & Toxicol, Jeddah 21589, Saudi Arabia
[4] King Abdulaziz Univ, Fac Pharm, Dept Pharmaceut, Jeddah 21589, Saudi Arabia
[5] King Abdulaziz Univ, Ctr Excellence Drug Res & Pharmaceut Ind, Jeddah 21589, Saudi Arabia
[6] King Abdulaziz Univ, Mohamed Saeed Tamer Chair Pharmaceut Ind, Jeddah 21589, Saudi Arabia
[7] German Univ Cairo, Fac Pharm & Biotechnol, Dept Pharmaceut Technol, Cairo 11835, Egypt
[8] King Abdulaziz Univ, Fac Pharm, Dept Nat Prod, Jeddah 21589, Saudi Arabia
[9] Suez Canal Univ, Fac Pharm, Dept Pharmacognosy, Ismailia 41522, Egypt
关键词
Teucrium polium; Chemical profiling; Chitosan; Nanogel; Angiogenesis; Diabetic wounds; TANDEM MASS-SPECTROMETRY; LIQUID-CHROMATOGRAPHY; PHENOLIC-COMPOUNDS; ELECTROSPRAY-IONIZATION; ANTIOXIDANT ACTIVITY; GROWTH-FACTOR; HPLC-MS/MS; L; PLASMA; FLAVONOIDS;
D O I
10.1016/j.biopha.2023.115626
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
Healing of wounds is the most deteriorating diabetic experience. Felty germander (Teucrium polium) possesses antioxidant, anti-inflammatory and antimicrobial activities that could accelerate wound healing. Further, nanohydrogels help quicken healing and are ideal biomaterials for drug delivery. In the current study, the chemical profiling, and standardization of T. polium methanolic extract by LC-ESI/TOF/MS/MS and quantitative HPLC-DAD analyses were achieved. The wound healing enhancement in diabetic rats by T. polium nano-preparation (TP-NP) as chitosan nanogel (CS-NG) and investigating the potential mechanisms were investigated. The prepared hydrogel-based TP-NP were characterized with respect to particle size, zeta potential, pH, viscosity, and release of major components. LC-ESI/TOF/MS/MS metabolomic profiling of T. polium revealed the richness of the plant with phenolic compounds, particularly flavonoids. In addition, several terpenoids were detected. Kaempferol content of T. polium was estimated to be 7.85 +/- 0.022 mg/ g of dry extract. The wound healing activity of TP-NP was explored in streptozotocin-induced diabetic rats. Diabetic animals were subjected to surgical wounding (1 cm diameter). Then they were divided in 5 groups (10 each). These included Group 1 (untreated control rats), Group 2 received the vehicle of CS-NG; Group 3 (0.5 g of TP prepared in hydrogel), Group 4 (0.5 g of TP-NP), Group 5 represented a positive control treated with 0.5 g of a commercial product. All treatments were applied topically for 21 days. Application of TP-NP on skin wounds of diabetic animals accelerated the healing process as evidenced by epithelium regeneration, formation of granulation tissue followed by epidermal proliferation, along with keratinization as verified by H&E. This was confirmed through enhanced collagen synthesis, as shown by raised hydroxyproline content and Col1A1 gene expression. Moreover, TP-NP significantly alleviated wound oxidative burst and diminished the expressions of inflammatory biomarkers. Meanwhile, TP-NP could enhance the expressions of transforming growth factor beta1 (TGF-beta 1), in addition to the angiogenic markers; vascular endothelia growth factor A (VEGFA) and platelet-derived growth factor receptor alpha (PDGFR alpha). Collectively, chitosan nanogel of T. polium accelerates wound healing in diabetic rats, which could be explained - at least partly - through alleviating oxidative stress and inflammation coupled with pro-angiogenic capabilities.
引用
收藏
页数:15
相关论文
共 116 条
[1]   Comparative Cytotoxic Evaluation of Zygophyllum album Root and Aerial Parts of Different Extracts and Their Biosynthesized Silver Nanoparticles on Lung A549 and Prostate PC-3 Cancer Cell Lines [J].
Abdelhameed, Reda F. A. ;
Nafie, Mohamed S. ;
Hal, Dina M. ;
Nasr, Ali M. ;
Swidan, Shady A. ;
Abdel-Kader, Maged S. ;
Ibrahim, Amany K. ;
Ahmed, Safwat A. ;
Badr, Jihan M. ;
Eltamany, Enas E. .
PHARMACEUTICALS, 2022, 15 (11)
[2]   A Comprehensive Characterisation of Rosemary tea Obtained from Rosmarinus officinalis L. Collected in a sub-Humid Area of Tunisia [J].
Achour, Mariem ;
Mateos, Raquel ;
Ben Fredj, Maha ;
Mtiraoui, Ali ;
Bravo, Laura ;
Saguem, Saad .
PHYTOCHEMICAL ANALYSIS, 2018, 29 (01) :87-100
[3]  
Akin M., 2010, Int. J. Pharm. Appl. Sci., V1, P55
[4]   Diabetes Mellitus in Saudi Arabia: A Review of the Recent Literature [J].
Al Dawish, Mohamed Abdulaziz ;
Robert, Asirvatham Alwin ;
Braham, Rim ;
Al Hayek, Ayman Abdallah ;
Al Saeed, Abdulghani ;
Ahmed, Rania Ahmed ;
Al Sabaan, Fahad Sulaiman .
CURRENT DIABETES REVIEWS, 2016, 12 (04) :359-368
[5]  
Alizadeh AM, 2011, IRAN J BASIC MED SCI, V14, P499
[6]   Incidence and prevalence rates of diabetes mellitus in Saudi Arabia: An overview [J].
Alotaibi, Abdulellah ;
Perry, Lin ;
Gholizadeh, Leila ;
Al-Ganmi, Ali .
JOURNAL OF EPIDEMIOLOGY AND GLOBAL HEALTH, 2017, 7 (04) :211-218
[7]   Phytochemical Screening, Antibacterial, Antifungal, Antiviral, Cytotoxic, and Anti-Quorum-Sensing Properties of Teucrium polium L. Aerial Parts Methanolic Extract [J].
Alreshidi, Mousa ;
Noumi, Emira ;
Bouslama, Lamjed ;
Ceylan, Ozgur ;
Veettil, Vajid N. ;
Adnan, Mohd ;
Danciu, Corina ;
Elkahoui, Salem ;
Badraoui, Riadh ;
Al-Motair, Khalid A. ;
Patel, Mitesh ;
De Feo, Vincenzo ;
Snoussi, Mejdi .
PLANTS-BASEL, 2020, 9 (11) :1-20
[8]   Teucrium polium L: An updated review of phytochemicals and biological activities [J].
Bahramikia, Seifollah ;
Gavyar, Parvaneh Hemmati Hassan ;
Yazdanparast, Razieh .
AVICENNA JOURNAL OF PHYTOMEDICINE, 2022, 12 (03) :224-240
[9]   Phytochemistry and Medicinal Properties of Teucrium polium L. (Lamiaceae) [J].
Bahramikia, Seifollah ;
Yazdanparast, Razieh .
PHYTOTHERAPY RESEARCH, 2012, 26 (11) :1581-1593
[10]  
Bancroft JD, 2008, Theory and Practice of Histological Techniques