Single-molecule tracking (SMT): a window into live-cell transcription biochemistry

被引：20
作者：
Dahal, Liza [1 ,2 ,3 ]
Walther, Nike [1 ,3 ]
Tjian, Robert [1 ,2 ,3 ]
Darzacq, Xavier [1 ,3 ]
Graham, Thomas G. W. [1 ,2 ,3 ]
机构：
[1] Univ Calif Berkeley, Dept Mol & Cell Biol, Berkeley, CA 94720 USA
[2] Univ Calif Berkeley, Howard Hughes Med Inst, Berkeley, CA 94720 USA
[3] Univ Calif Berkeley, Li Ka Shing Ctr Biomed & Hlth Sci, Berkeley, CA 94720 USA
来源：
BIOCHEMICAL SOCIETY TRANSACTIONS | 2023年 / 51卷 / 02期
基金：
美国国家卫生研究院;
关键词：
FLUORESCENCE COMPLEMENTATION BIFC; LOW-DENSITY-LIPOPROTEIN; IN-VIVO; SUPERRESOLUTION MICROSCOPY; FACTOR DYNAMICS; SCMOS CAMERA; BINDING; LOCALIZATION; PROTEINS; TAG;
D O I：
10.1042/BST20221242
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
How molecules interact governs how they move. Single-molecule tracking (SMT) thus provides a unique window into the dynamic interactions of biomolecules within live cells. Using transcription regulation as a case study, we describe how SMT works, what it can tell us about molecular biology, and how it has changed our perspective on the inner workings of the nucleus. We also describe what SMT cannot yet tell us and how new technical advances seek to overcome its limitations. This ongoing progress will be imperative to address outstanding questions about how dynamic molecular machines function in live cells.
引用
收藏
页码：557 / 569
页数：13
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