G protein-coupled bile acid receptor 1 reduced hepatic immune response and inhibited NFκB, PI3K/AKT, and PKC/P38 MAPK signaling pathway in hybrid grouper

被引：1
作者：
Xu, Jia [1 ,2 ]
Cao, Junming [1 ,3 ,4 ]
Tan, Beiping [1 ,4 ]
Xie, Shiwei [1 ,3 ,4 ]
机构：
[1] Guangdong Ocean Univ, Fisheries Coll, Lab Aquat Anim Nutr & Feed, Zhanjiang, Peoples R China
[2] Guangxi Acad Sci, Guangxi Acad Marine Sci, Guangxi Key Lab Marine Environm Sci, Nanning, Peoples R China
[3] Minist Agr, Key Lab Aquat Livestock & Poultry Feed Sci & Techn, Zhanjiang, Peoples R China
[4] Aquat Anim Precis Nutr High Efficiency Feed Engn R, Zhanjiang, Peoples R China
来源：
JOURNAL OF ANIMAL SCIENCE | 2023年 / 101卷
基金：
中国国家自然科学基金; 国家重点研发计划;
关键词：
fish; inflammatory responses; NF kappa B; PI3K/AKT; TGR5; INFLAMMATORY RESPONSE; LIVER-DISEASES; TGR5; AGONISTS; CELLS; FXR; POTENT;
D O I：
10.1093/jas/skad307
中图分类号：
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号：
0905 ;
摘要：
The mammalian G protein-coupled bile acid receptor 1 (TGR5) is involved in the inflammatory response. However, the functions of TGR5 in the immune response of fish remain unclear. In this study, the full-length sequence of tgr5 from hybrid grouper (Epinephelus fuscoguttatus female x E. lanceolatus male) was cloned, and the function of TGR5 in the immune response was explored. The results showed that the ORF of tgr5 gene in hybrid grouper was 1029 bp and encoded 342 amino acids. Activation of TGR5 by INT-777 significantly decreased the activities and mRNA expression of TNF alpha and IL1 beta, whereas inhibition of TGR5 by SBI-115 showed the opposite effect. SBI-115 treatment significantly increased the expression of phosphorylated inhibitor kappa B alpha (p-IKB alpha) protein. After the INT-777 treatment, the concentration of protein kinase C (PKC) and expression of the p38 mitogen-activated protein kinases (p38a), p38b and p38c, were significantly decreased in vivo. INT-777 agonist significantly decreased the expression of phosphorylated phosphoinositide 3-kinase (p-PI3K) protein and the ratio of phosphorylated and nonphosphorylated serine/threonine-protein kinase (p-AKT/AKT). In conclusion, activation of hepatic TGR5 inhibited the PKC/P38 MAPK, PI3K/AKT, NF kappa B signaling pathway and improved hepatic immune responses of hybrid grouper in vivo and in vitro.
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页数：15
相关论文
共 64 条
  • [1] An W.Q., 2018, J GUANGDONG OCEAN U, V38, P8, DOI [10.3969/j.issn.1673-9159.2018.04.002, DOI 10.3969/J.ISSN.1673-9159.2018.04.002]
  • [2] Anwer Mohammed Sawkat, 2012, J Biosci (Rajshari), V20, P1
  • [3] Chao L., 2017, J GUANGDONG OCEAN U, V37, P7, DOI DOI 10.3969/J.ISSN.1673-9159.2017.03.001
  • [4] Chao-Fan Z., 2018, J. Guangdong Ocean Univer, DOI [10.3969/j.issn.1673-9159.2018.05.005, DOI 10.3969/J.ISSN.1673-9159.2018.05.005]
  • [5] Chen Q., 2016, Journal of Guangdong Ocean University, V36, P35, DOI [DOI 10.3969/J.ISSN.1673-9159.2016.01.007, 10.3969/j.issn.1673-9159.2016.01.007, DOI 10.3969/J.ISSN.1673-9159.2016]
  • [6] Chen Y.Y., 2015, J. Guangdong Ocean Univ., V35, P35, DOI [10.3969/j.issn.1673-9159.2015.06.007Vol.35, DOI 10.3969/J.ISSN.1673-9159.2015.06.007VOL.35]
  • [7] Bile acid receptors FXR and TGR5 signaling in fatty liver diseases an therapy
    Chiang, John Y. L.
    Ferrell, Jessica M.
    [J]. AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY, 2020, 318 (03): : G554 - G573
  • [8] Intestinal microbiota in fishes: what's known and what's not
    Clements, Kendall D.
    Angert, Esther R.
    Montgomery, W. Linn
    Choat, J. Howard
    [J]. MOLECULAR ECOLOGY, 2014, 23 (08) : 1891 - 1898
  • [9] NF-κB and the link between inflammation and cancer
    DiDonato, Joseph A.
    Mercurio, Frank
    Karin, Michael
    [J]. IMMUNOLOGICAL REVIEWS, 2012, 246 : 379 - 400
  • [10] Eggink H M., 2014, International Journal of Interferon, Cytokine and Mediator Research, V6, P27, DOI DOI 10.2147/IJICMR.S40102
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