Weighted Gene Co-Expression Network Analysis to Explore Hub Genes of Resveratrol Biosynthesis in Exocarp and Mesocarp of 'Summer Black' Grape

被引：7

作者：

Li, Chengyue ^{[1
]}

Chen, Lifang ^{[1
]}

Fan, Quan ^{[1
]}

He, Pengfei ^{[1
]}

Wang, Congqiao ^{[1
]}

Huang, Huaxing ^{[1
]}

Huang, Ruyan ^{[1
]}

Tang, Jiaqi ^{[1
]}

Tadda, Shehu A. ^{[2
]}

Qiu, Dongliang ^{[1
]}

Qiu, Zhipeng ^{[3
]}

机构：

[1] Fujian Agr & Forestry Univ, Coll Hort, Fuzhou 350002, Peoples R China

[2] Fed Univ Dutsin Ma, Fac Agr, Dept Agron, Dutsin Ma 821101, Nigeria

[3] Lunong Agr Technol Co Ltd, Xiamen 361100, Peoples R China

来源：

PLANTS-BASEL | 2023年 / 12卷 / 03期

关键词：

grapes; resveratrol biosynthesis; RNA-seq; WGCNA; hub genes; STILBENE SYNTHASE GENE; PHENYLALANINE AMMONIA-LYASE; CHALCONE SYNTHASES; MOLECULAR ANALYSIS; TRANS-RESVERATROL; BERRY; EXPRESSION; HYDROXYCINNAMOYLTRANSFERASE; LOCALIZATION; COENZYME;

D O I：

10.3390/plants12030578

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Resveratrol is a polyphenol compound beneficial to human health, and its main source is grapes. In the present study, the molecular regulation of resveratrol biosynthesis in developing grape berries was investigated using weighted gene co-expression network analysis (WGCNA). At the same time, the reason for the resveratrol content difference between grape exocarp (skin) and mesocarp (flesh) was explored. Hub genes (CHS, STS, F3'5'H, PAL, HCT) related to resveratrol biosynthesis were screened with Cytoscape software. The expression level of hub genes in the exocarp was significantly higher than that in the mesocarp, and the expressions of the hub genes and the content of resveratrol in exocarp peaked at the maturity stage. While the expression levels of PAL, CHS and STS in the mesocarp, reached the maximum at the maturity stage, and F3 ' 5 ' H and HCT decreased. These hub genes likely play a key role in resveratrol biosynthesis. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis further indicated that resveratrol biosynthesis was related to flavonoid biosynthesis, phenylalanine metabolism, phenylpropanoid biosynthesis, and stilbene biosynthesis pathways. This study has theoretical significance for exploring genes related to resveratrol biosynthesis in the exocarp and mesocarp of grapes, and provides a theoretical basis for the subsequent function and regulatory mechanism of hub genes.

引用

页数：16

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