Ex Vivo Osteogenesis Induced by Calcium Silicate-Based Cement Extracts

Calcium silicate-based cements are used in a variety of clinical conditions affecting the pulp tissue, relying on their inductive effect on tissue mineralization. This work aimed to evaluate the biological response of calcium silicate-based cements with distinct properties-the fast-setting Biodentine (TM) and TotalFill (R) BC RRMT Fast Putty, and the classical slow-setting ProRoot (R) MTA, in an ex vivo model of bone development. Briefly, eleven-day-old embryonic chick femurs were cultured for 10 days in organotypic conditions, being exposed to the set cements' eluates and, at the end of the culture period, evaluated for osteogenesis/bone formation by combining microtomographic analysis and histological histomorphometric assessment. ProRoot (R) MTA and TotalFill (R) extracts presented similar levels of calcium ions, although significantly lower than those released from Biodentine (TM). All extracts increased the osteogenesis/tissue mineralization, assayed by microtomographic (BV/TV) and histomorphometric (% of mineralized area; % of total collagen area, and % of mature collagen area) indexes, although displaying distinct dose-dependent patterns and quantitative values. The fast-setting cements displayed better performance than that of ProRoot (R) MTA, with Biodentine (TM) presenting the best performance, within the assayed experimental model.