Fluorescence-Enhanced Microfluidic Biosensor Platform Based on Magnetic Beads with Highly Stable ZnO Nanorods for Biomarker Detection

被引:19
作者
Meeseepong, Montri [1 ]
Ghosh, Gargi [2 ]
Shrivastava, Sajal [3 ]
Lee, Nae-Eung [1 ,4 ,5 ]
机构
[1] Sungkyunkwan Univ, SKKU Adv Inst Nanotechnol St, Suwon 16419, Gyeonggi Do, South Korea
[2] Sungkyunkwan Univ, Sch Adv Mat Sci & Engn, Suwon 16419, Gyeonggi Do, South Korea
[3] Symbiosis Int Univ, Symbiosis Ctr Nanosci & Nanotechnol, Pune 412115, India
[4] Sungkyunkwan Univ, Samsung Adv Inst Hlth Sci & Technol SAIHST, Inst Quantum Biophys IQB, Suwon 16419, Gyeonggi Do, South Korea
[5] Sungkyunkwan Univ, Biomed Inst Convergence SKKU BICS, Suwon 16419, Gyeonggi Do, South Korea
基金
新加坡国家研究基金会;
关键词
ZnO enhanced fluorescence; movable sensors; 3D biosensors; fluorescence biosensors; aptasensing; LABEL-FREE; IMMUNOASSAY; CHIP;
D O I
10.1021/acsami.2c22352
中图分类号
TB3 [工程材料学];
学科分类号
0805 ; 080502 ;
摘要
Existing affinity-based fluorescence biosensing systems for monitoring of biomarkers often utilize a fixed solid substrate immobilized with capture probes limiting their use in continuous or intermittent biomarker detection. Furthermore, there have been challenges of integrating fluorescence biosensors with a microfluidic chip and low-cost fluorescence detector. Herein, we demonstrated a highly efficient and movable fluorescence-enhanced affinity-based fluorescence biosensing platform that can overcome the current limitations by combining fluorescence enhancement and digital imaging. Fluorescence-enhanced movable magnetic beads (MBs) decorated with zinc oxide nanorods (MB-ZnO NRs) were used for digital fluorescence-imaging-based aptasensing of biomolecules with improved signal-to-noise ratio. High stability and homogeneous dispersion of photostable MB-ZnO NRs were obtained by grafting bilayered silanes onto the ZnO NRs. The ZnO NRs formed on MB significantly improved the fluorescence signal up to 2.35 times compared to the MB without ZnO NRs. Moreover, the integration of a microfluidic device for flow-based biosensing enabled continuous measurements of biomarkers in an electrolytic environment. The results showed that highly stable fluorescence-enhanced MB-ZnO NRs integrated with a microfluidic platform have significant potential for diagnostics, biological assays, and continuous or intermittent biomonitoring.
引用
收藏
页码:21754 / 21765
页数:12
相关论文
共 54 条
[1]   Ultrasensitive detection of cytokines enabled by nanoscale ZnO arrays [J].
Adalsteinsson, Viktor ;
Parajuli, Omkar ;
Kepics, Stephen ;
Gupta, Abhishek ;
Reeves, W. Brian ;
Hahm, Jong-in .
ANALYTICAL CHEMISTRY, 2008, 80 (17) :6594-6601
[2]   Microfluidic integration of regeneratable electrochemical affinity-based biosensors for continual monitoring of organ-on-a-chip devices [J].
Aleman, Julio ;
Kilic, Tugba ;
Mille, Luis S. ;
Shin, Su Ryon ;
Zhang, Yu Shrike .
NATURE PROTOCOLS, 2021, 16 (05) :2564-2593
[3]   Detection of exosomes by ZnO nanowires coated three-dimensional scaffold chip device [J].
Chen, Zhen ;
Cheng, Shi-Bo ;
Cao, Pan ;
Qiu, Quan-Fa ;
Chen, Yan ;
Xie, Min ;
Xu, Yu ;
Huang, Wei-Hua .
BIOSENSORS & BIOELECTRONICS, 2018, 122 :211-216
[4]   Reusable smartphone-facilitated mobile fluorescence biosensor for rapid and sensitive on-site quantitative detection of trace pollutants [J].
Cheng, Yuan ;
Wang, Hongliang ;
Zhuo, Yuxin ;
Song, Dan ;
Li, Chunsheng ;
Zhu, Anna ;
Long, Feng .
BIOSENSORS & BIOELECTRONICS, 2022, 199
[5]   Approaching near real-time biosensing: Microfluidic microsphere based biosensor for real-time analyte detection [J].
Cohen, Noa ;
Sabhachandani, Pooja ;
Golberg, Alexander ;
Konry, Tania .
BIOSENSORS & BIOELECTRONICS, 2015, 66 :454-460
[6]   Heart-on-a-chip platforms and biosensor integration for disease modeling and phenotypic drug screening [J].
Criscione, Joseph ;
Rezaei, Zahra ;
Cantu, Carol M. Hernandez ;
Murphy, Sean ;
Shin, Su Ryon ;
Kim, Deok-Ho .
BIOSENSORS & BIOELECTRONICS, 2023, 220
[7]   An antifouling coating that enables affinity-based electrochemical biosensing in complex biological fluids [J].
del Rio, Jonathan Sabate ;
Henry, Olivier Y. F. ;
Jolly, Pawan ;
Ingber, Donald E. .
NATURE NANOTECHNOLOGY, 2019, 14 (12) :1143-+
[8]   Label-free biodetection using a smartphone [J].
Gallegos, Dustin ;
Long, Kenneth D. ;
Yu, Hojeong ;
Clark, Peter P. ;
Lin, Yixiao ;
George, Sherine ;
Nath, Pabitra ;
Cunningham, Brian T. .
LAB ON A CHIP, 2013, 13 (11) :2124-2132
[9]   Wash-free magnetic immunoassay of the PSA cancer marker using SERS and droplet microfluidics [J].
Gao, Rongke ;
Cheng, Ziyi ;
deMello, Andrew J. ;
Choo, Jaebum .
LAB ON A CHIP, 2016, 16 (06) :1022-1029
[10]   Fluorescence-enhanced microfluidic sensor for highly sensitive in-situ detection of copper ions in lubricating oil [J].
Gao, Yuefeng ;
Pan, Xinxiang ;
Xu, Sai ;
Liu, Zhijian ;
Wang, Junsheng ;
Yu, Kezhen ;
Wang, Chengfa ;
Yuan, Haichao ;
Wu, Sen .
MATERIALS & DESIGN, 2020, 191 (191)