Engineering and Development of a Tissue Model for the Evaluation of Microneedle Penetration Ability, Drug Diffusion, Photothermal Activity, and Ultrasound Imaging: A Promising Surrogate to Ex Vivo and In Vivo Tissues

被引：27

作者：

Makvandi, Pooyan ^{[1
,2
]}

Shabani, Majid ^{[3
,4
]}

Rabiee, Navid ^{[5
,14
]}

Anjani, Qonita Kurnia ^{[6
]}

Maleki, Aziz ^{[7
]}

Zare, Ehsan Nazarzadeh ^{[8
]}

Bin Sabri, Akmal Hidayat ^{[6
]}

De Pasquale, Daniele ^{[1
]}

Koskinopoulou, Maria ^{[9
]}

Sharifi, Esmaeel ^{[10
]}

Sartorius, Rossella ^{[11
]}

Seyedhamzeh, Mohammad ^{[7
]}

Bochani, Shayesteh ^{[7
]}

Hirata, Ikue ^{[1
]}

Paiva-Santos, Ana Claudia ^{[12
,13
]}

Mattos, Leonardo S. ^{[9
]}

Donnelly, Ryan F. ^{[6
]}

Mattoli, Virgilio ^{[1
]}

机构：

[1] Ist Italiano Tecnol, Ctr Mat Interfaces, Viale Rinaldo Piaggio 34, I-56025 Pontedera, Pisa, Italy

[2] Univ Edinburgh, Inst Bioengn, Sch Engn, Edinburgh EH9 3JL, Scotland

[3] Scuola Super Sant Anna, BioRobot Inst, I-56025 Pontedera, Pisa, Italy

[4] Ist Italiano Tecnol, Bioinspired Soft Robot Lab, I-16163 Genoa, Italy

[5] Murdoch Univ, Ctr Mol Med & Innovat Therapeut, Perth, WA 6150, Australia

[6] Queens Univ Belfast, Sch Pharm, 97 Lisburn Rd, Belfast BT9 7BL, North Ireland

[7] Zanjan Univ Med Sci, Nanotechnol Res Ctr ZPNRC, Sch Pharm, Dept Pharmaceut Nanotechnol, Zanjan 4513956184, Iran

[8] Damghan Univ, Sch Chem, Damghan 3671645667, Iran

[9] Ist Italiano Tecnol, Dept Adv Robot ADVR, I-16163 Genoa, Italy

[10] Hamadan Univ Med Sci, Canc Res Ctr, Hamadan 6517838736, Iran

[11] Natl Res Council CNR, Inst Biochem & Cell Biol IBBC, I-80131 Naples, Italy

[12] Univ Coimbra, Fac Pharm, Dept Pharmaceut Technol, P-3000548 Coimbra, Portugal

[13] Univ Coimbra, Fac Pharm, Dept Pharmaceut Technol, LAQV,REQUIMTE, P-3000548 Coimbra, Portugal

[14] Macquarie Univ, Sch Engn, Sydney, NSW 2109, Australia

来源：

ADVANCED MATERIALS | 2023年 / 35卷 / 18期

关键词：

drug delivery models; drug deposition; drug release profiles; microneedle patch penetration; photothermal activity; skin model; tissue models; OPTICAL COHERENCE TOMOGRAPHY; STRATUM-CORNEUM; SKIN MODELS; MEMBRANE; INSIGHT; TOOL;

D O I：

10.1002/adma.202210034

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Driven by regulatory authorities and the ever-growing demands from industry, various artificial tissue models have been developed. Nevertheless, there is no model to date that is capable of mimicking the biomechanical properties of the skin whilst exhibiting the hydrophilicity/hydrophobicity properties of the skin layers. As a proof-of-concept study, tissue surrogates based on gel and silicone are fabricated for the evaluation of microneedle penetration, drug diffusion, photothermal activity, and ultrasound bioimaging. The silicone layer aims to imitate the stratum corneum while the gel layer aims to mimic the water-rich viable epidermis and dermis present in in vivo tissues. The diffusion of drugs across the tissue model is assessed, and the results reveal that the proposed tissue model shows similar behavior to a cancerous kidney. In place of typical in vitro aqueous solutions, this model can also be employed for evaluating the photoactivity of photothermal agents since the tissue model shows a similar heating profile to skin of mice when irradiated with near-infrared laser. In addition, the designed tissue model exhibits promising results for biomedical applications in optical coherence tomography and ultrasound imaging. Such a tissue model paves the way to reduce the use of animals testing in research whilst obviating ethical concerns.

引用

页数：20

共 57 条

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