Multiplex One-Step RT-qPCR Assays for Simultaneous Detection of SARS-CoV-2 and Other Enteric Viruses of Dogs and Cats

被引：11

作者：

Thieulent, Come J. ^{[1
,2
]}

Carossino, Mariano ^{[1
,2
]}

Peak, Laura ^{[1
]}

Wolfson, Wendy ^{[3
]}

Balasuriya, Udeni B. R. ^{[1
,2
]}

机构：

[1] Louisiana State Univ, Sch Vet Med, Louisiana Anim Dis Diagnost Lab, Baton Rouge, LA 70803 USA

[2] Louisiana State Univ, Sch Vet Med, Dept Pathobiol Sci, Baton Rouge, LA 70803 USA

[3] Louisiana State Univ, Sch Vet Med, Dept Vet Clin Sci, Baton Rouge, LA 70803 USA

来源：

VIRUSES-BASEL | 2023年 / 15卷 / 09期

关键词：

reverse transcription-qPCR (RT-qPCR); multiplex; feline enteric virus; canine enteric virus; SARS-CoV-2; diagnostics; zoonosis; reverse zoonosis; REAL-TIME PCR; FELINE PANLEUKOPENIA VIRUS; CANINE PARVOVIRUS; ROTAVIRUS STRAIN; DIARRHEA; PATHOGENESIS; QUANTITATION; INFECTION; TRANSMISSION; ORIGIN;

D O I：

10.3390/v15091890

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) was transmitted from humans to dogs and cats (reverse zoonosis) during the COVID-19 pandemic. SARS-CoV-2 has been detected in fecal samples of infected dogs and cats, indicating potential fecal-oral transmission, environmental contamination, and zoonotic transmission (i.e., spillback). Additionally, gastrointestinal viral infections are prevalent in dogs and cats. In this study, we developed and validated a panel of multiplex one-step reverse transcription-quantitative polymerase chain reaction (RT-qPCR) assays for the simultaneous detection of SARS-CoV-2 and common canine enteric viruses: Canine Enteric Assay_1 (CEA_1) for the detection of canine adenovirus-1, canine enteric coronavirus, canine distemper virus, and canine parvovirus, and CEA_2 for the detection of rotavirus A (RVA), and SARS-CoV-2); or common feline enteric viruses (Feline Enteric Assay_1 (FEA_1) for the detection of feline enteric coronavirus, feline panleukopenia virus, RVA, and SARS-CoV-2). All assays demonstrated high analytical sensitivity, detecting as few as 5-35 genome copies/mu L in multiplex format. The repeatability and reproducibility of the multiplex assays were excellent, with coefficient of variation <4%. Among the 58 clinical samples tested, 34.5% were positive for at least one of these viruses, and SARS-CoV-2 was detected in two samples collected from one dog and one cat, respectively. In conclusion, these newly developed one-step multiplex RT-qPCR assays allow for rapid diagnosis of enteric viral infections, including SARS-CoV-2, in dogs and cats.

引用

页数：14

共 68 条

[11] Presence of infectious agents and co-infections in diarrheic dogs determined with a real-time polymerase chain reaction-based panel [J].

da Rocha Gizzi, Aline Baumann ;

Oliveira, Simone Tostes ;

Leutenegger, Christian M. ;

Estrada, Marko ;

Kozemjakin, Denise Adamczyk ;

Stedile, Rafael ;

Marcondes, Mary ;

Biondo, Alexander Welker .

BMC VETERINARY RESEARCH, 2014, 10

[12] Rare AU-1-Like G3P[9] human rotaviruses with a Kun-like NSP4 gene detected in children with diarrhea in Italy [J].

De Grazia, S. ;

Giammanco, G. M. ;

Martella, V. ;

Ramirez, S. ;

Colomba, C. ;

Cascio, A. ;

Arista, S. .

JOURNAL OF CLINICAL MICROBIOLOGY, 2008, 46 (01) :357-360

[13] Canine-origin G3P[3] rotavirus strain in child with acute gastroenteritis [J].

De Grazia, Simona ;

Martella, Vito ;

Giammanco, Giovanni M. ;

Gomara, Miren Iturriza ;

Ramirez, Stefania ;

Cascio, Antonio ;

Colomba, Claudia ;

Arista, Serenella .

EMERGING INFECTIOUS DISEASES, 2007, 13 (07) :1091-1093

[14] Infection of SARS-CoV-2 in domestic dogs associated with owner viral load [J].

de Souza Barbosa, Altina Bruna ;

Kmetiuk, Louise Bach ;

de Carvalho, Otavio Valerio ;

Drulla Brandao, Ana Perola ;

Doline, Fernando Rodrigo ;

Rangel Silveira Lopes, Sibele Rejane ;

Meira, Douglas Adamoski ;

de Souza, Emanuel Maltempi ;

Trindade, Edvaldo da Silva ;

Baura, Valter ;

Barbosa, David Soeiro ;

Brandespim, Daniel Friguglietti ;

Galhardo, Juliana Arena ;

Pettan-Brewer, Christina ;

de Sa Guimaraes, Ana Marcia ;

Dutra, Valeria ;

de Morais, Helio Autran ;

Huergo, Luciano ;

dos Santos, Andrea Pires ;

Biondo, Alexander Welker .

RESEARCH IN VETERINARY SCIENCE, 2022, 153 :61-65

[15] A real-time PCR assay for rapid detection and quantitation of canine parvovirus type 2 in the feces of dogs [J].

Decaro, N ;

Elia, G ;

Martella, V ;

Desario, C ;

Campolo, M ;

Di Trani, L ;

Tarsitano, E ;

Tempesta, M ;

Buonavoglia, C .

VETERINARY MICROBIOLOGY, 2005, 105 (01) :19-28

[16] Quantitation of canine coronavirus RNA in the faeces of dogs by TaqMan RT-PCR [J].

Decaro, N ;

Pratelli, A ;

Campolo, M ;

Elia, G ;

Martella, V ;

Tempesta, M ;

Buonavoglia, C .

JOURNAL OF VIROLOGICAL METHODS, 2004, 119 (02) :145-150

[17] Canine parvovirus-A review of epidemiological and diagnostic aspects, with emphasis on type 2c [J].

Decaro, Nicola ;

Buonavoglia, Canio .

VETERINARY MICROBIOLOGY, 2012, 155 (01) :1-12

[18] Western European epidemiological survey for parvovirus and coronavirus infections in dogs [J].

Decaro, Nicola ;

Desario, Costantina ;

Billi, Monica ;

Mari, Viviana ;

Elia, Gabriella ;

Cavalli, Alessandra ;

Martella, Vito ;

Buonavoglia, Canio .

VETERINARY JOURNAL, 2011, 187 (02) :195-199

[19] A multiplex PCR method for the simultaneous detection of three viruses associated with canine viral enteric infections [J].

Deng, Xiaoyu ;

Zhang, Jiali ;

Su, Jiazi ;

Liu, Hao ;

Cong, Yanlong ;

Zhang, Lei ;

Zhang, Kemeng ;

Shi, Ning ;

Lu, Rongguang ;

Yan, Xijun .

ARCHIVES OF VIROLOGY, 2018, 163 (08) :2133-2138

[20] A duplex real-time PCR assay based on TaqMan technology for simultaneous detection and differentiation of canine adenovirus types 1 and 2 [J].

Dowgier, Giulia ;

Mari, Viviana ;

Losurdo, Michele ;

Larocca, Vittorio ;

Colaianni, Maria Loredana ;

Cirone, Francesco ;

Lucente, Maria Stella ;

Martella, Vito ;

Buonavoglia, Canio ;

Decaro, Nicola .

JOURNAL OF VIROLOGICAL METHODS, 2016, 234 :1-6

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