Bioinspired multifunctional injectable hydrogel for hemostasis and infected wound management

被引:59
作者
Fang, Wen [1 ]
Yang, Ling [2 ]
Chen, Yihao [3 ]
Hu, Qiaoling [1 ]
机构
[1] Zhejiang Univ, Dept Polymer Sci & Engn, MOE Key Lab Macromol Synth & Functionalizat, Hangzhou 310027, Peoples R China
[2] Inst Flexible Elect Technol THU, Jiaxing Key Lab Flexible Elect based Intelligent S, Jiaxing, Peoples R China
[3] Beihang Univ, Sch Engn Med, Beijing, Peoples R China
基金
中国国家自然科学基金;
关键词
Multifunctional; Injectable hydrogel; Hemostasis; Infected wound management; Broad-spectrum antibacterial; TISSUE ADHESIVES; CHITOSAN; SKIN;
D O I
10.1016/j.actbio.2023.01.021
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
Routine wound management faces significant challenges including rebleeding and bacterial infection that affect millions of people each year. However, conventional wound dressings (e.g., gauze, bandage) are limited to simply cover the injured surfaces and rarely show special functionality to promote the wound recovery. Currently, injectable hydrogels have been widely designed as multifunctional wound dressings to manage the hemostatic and wound healing process. Nevertheless, the integration of multiple functions through simple composition and easy construction is still difficult and hardly achieved. Herein, we re-ported a bioinspired multifunctional injectable hydrogel (CQCS@gel) consisted of only two components, catechol-functionalized quaternized chitosan (CQCS) and dibenzaldehyde-terminated poly(ethylene gly-col) (DB-PEG20 0 0). The building blocks endowed CQCS@gel with tissue-adhesive, antibacterial, antioxidant, self-healing and pH-responsive properties. Based on the in vivo hemostatic study, quick hemostasis for acute tissue injuries such as liver and carotid wounds was realized owing to the rapid gelation rate and strong tissue-adhesiveness of CQCS@gel. Moreover, CQCS@gel remarkably boosted the chronic recovery process of MRSA-infected cutaneous wounds by promoting collagen deposition, hair follicles regeneration and angiogenesis. Overall, this multifunctional injectable hydrogel shows potentials as a universal wound dressing in clinical applications, enabling both hemostasis and infected wound management.Statement of significanceThis is the first report showing the multifunctional injectable hydrogel (CQCS@gel) consisted of catechol-functionalized quaternized chitosan and dibenzaldehyde-terminated poly(ethylene glycol). The incorpora-tion of quaternary ammonium groups imparted the CQCS@gel with outstanding contact-active bacterial killing efficiency and the catechol moieties enhanced its tissue adhesive and antioxidant properties. More-over, the reversible imine crosslinks endowed the CQCS@gel with self-healing and pH-responsive drug release capabilities. These multiple functions were integrated into a single injectable hydrogel system with easy availability and low cost. In vitro and in vivo results showed that the newly designed hydrogel was biocompatible, realized successful sealing hemostasis under multiple bleeding scenarios and enabled accelerated healing of infected skin wounds.(c) 2023 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.
引用
收藏
页码:50 / 66
页数:17
相关论文
共 52 条
[1]   Self-defensive antibiotic-loaded layer-by-layer coatings: Imaging of localized bacterial acidification and pH-triggering of antibiotic release [J].
Albright, Victoria ;
Zhuk, Iryna ;
Wang, Yuhao ;
Selin, Victor ;
van de Belt-Gritter, Betsy ;
Busscher, Henk J. ;
van der Mei, Henny C. ;
Sukhishvili, Svetlana A. .
ACTA BIOMATERIALIA, 2017, 61 :66-74
[2]   Chitosan and Cellulose-Based Hydrogels for Wound Management [J].
Alven, Sibusiso ;
Aderibigbe, Blessing Atim .
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2020, 21 (24) :1-30
[3]   Antimicrobial activity of catechol functionalized-chitosan versus Staphylococcus epidermidis [J].
Amato, Andrea ;
Migneco, Luisa Maria ;
Martinelli, Andrea ;
Pietrelli, Loris ;
Piozzi, Antonella ;
Francolini, Iolanda .
CARBOHYDRATE POLYMERS, 2018, 179 :273-281
[4]   Aldehyde-Amine Chemistry Enables Modulated Biosealants with Tissue-Specific Adhesion [J].
Artzi, Natalie ;
Shazly, Tarek ;
Baker, Aaron B. ;
Bon, Adriana ;
Edelman, Elazer R. .
ADVANCED MATERIALS, 2009, 21 (32-33) :3399-+
[5]   Bioinspired mechanically active adhesive dressings to accelerate wound closure [J].
Blacklow, S. O. ;
Li, J. ;
Freedman, B. R. ;
Zeidi, M. ;
Chen, C. ;
Mooney, D. J. .
SCIENCE ADVANCES, 2019, 5 (07)
[6]   Bioinspired Multifunctional Hybrid Hydrogel Promotes Wound Healing [J].
Chen, Guopu ;
Yu, Yunru ;
Wu, Xiuwen ;
Wang, Gefei ;
Ren, Jianan ;
Zhao, Yuanjin .
ADVANCED FUNCTIONAL MATERIALS, 2018, 28 (33)
[7]  
Dai TH, 2011, EXPERT REV ANTI-INFE, V9, P857, DOI [10.1586/eri.11.59, 10.1586/ERI.11.59]
[8]   In situ forming injectable hydrogels for drug delivery and wound repair [J].
Dimatteo, Robert ;
Darling, Nicole J. ;
Segura, Tatiana .
ADVANCED DRUG DELIVERY REVIEWS, 2018, 127 :167-184
[9]   Challenges in the management of chronic wound infections [J].
Falcone, Marco ;
De Angelis, Barbara ;
Pea, Federico ;
Scalise, Alessandro ;
Stefani, Stefania ;
Tasinato, Rolando ;
Zanetti, Orazio ;
Dalla Paola, Luca .
JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE, 2021, 26 :140-147
[10]   Injectable, Intrinsically Antibacterial Conductive Hydrogels with Self-Healing and pH Stimulus Responsiveness for Epidermal Sensors and Wound Healing [J].
Fan, Ling ;
He, Zhongjie ;
Peng, Xueliang ;
Xie, Jinliang ;
Su, Fangfang ;
Wei, Dai-Xu ;
Zheng, Yaping ;
Yao, Dongdong .
ACS APPLIED MATERIALS & INTERFACES, 2021, 13 (45) :53541-53552