From femtoseconds to minutes: time-resolved macromolecular crystallography at XFELs and synchrotrons

被引：14

作者：

Caramello, Nicolas ^{[1
,2
]}

Royant, Antoine ^{[1
,3
]}

机构：

[1] European Synchrotron Radiat Facil, Struct Biol Grp, 1 Ave Martyrs,CS 40220, F-38043 Grenoble 9, France

[2] Univ Hamburg, Hamburg Ctr Ultrafast Imaging, HARBOR, Luruper Chaussee 149, D-22761 Hamburg, Germany

[3] Univ Grenoble Alpes, CNRS, Inst Biol Struct IBS, CEA, 71 Ave Martyrs,CS 10090, F-38044 Grenoble 9, France

来源：

ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY | 2024年 / 80卷

关键词：

time-resolved serial crystallography; synchrotrons; XFELs; structural photobiology; reaction-intermediate states; bacteriorhodopsin; cryo-trapping; X-RAY-RADIATION; INDUCED STRUCTURAL-CHANGES; BLUE-LIGHT PHOTORECEPTOR; ROOM-TEMPERATURE; SERIAL CRYSTALLOGRAPHY; ABSORPTION-SPECTROSCOPY; ENZYME CATALYSIS; BINDING DOMAIN; PROTEIN; BACTERIORHODOPSIN;

D O I：

10.1107/S2059798323011002

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Over the last decade, the development of time-resolved serial crystallography (TR-SX) at X-ray free-electron lasers (XFELs) and synchrotrons has allowed researchers to study phenomena occurring in proteins on the femtosecond-tominute timescale, taking advantage of many technical and methodological breakthroughs. Protein crystals of various sizes are presented to the X-ray beam in either a static or a moving medium. Photoactive proteins were naturally the initial systems to be studied in TR-SX experiments using pump-probe schemes, where the pump is a pulse of visible light. Other reaction initiations through small-molecule diffusion are gaining momentum. Here, selected examples of XFEL and synchrotron time-resolved crystallography studies will be used to highlight the specificities of the various instruments and methods with respect to time resolution, and are compared with cryo-trapping studies.

引用

页码：60 / 79

页数：20

共 145 条

[61] CRYOCRYSTALLOGRAPHY OF RIBOSOMAL PARTICLES [J].

HOPE, H ;

FROLOW, F ;

VONBOHLEN, K ;

MAKOWSKI, I ;

KRATKY, C ;

HALFON, Y ;

DANZ, H ;

WEBSTER, P ;

BARTELS, KS ;

WITTMANN, HG ;

YONATH, A .

ACTA CRYSTALLOGRAPHICA SECTION B-STRUCTURAL SCIENCE CRYSTAL ENGINEERING AND MATERIALS, 1989, 45 :190-199

[62]

Hutchison CDM, 2023, NAT CHEM, V15, DOI 10.1038/s41557-023-01275-1

[63]

Jung YO, 2013, NAT CHEM, V5, P212, DOI [10.1038/NCHEM.1565, 10.1038/nchem.1565]

[64] Structures of the intermediates of Kok's photosynthetic water oxidation clock [J].

Kern, Jan ;

Chatterjee, Ruchira ;

Young, Iris D. ;

Fuller, Franklin D. ;

Lassalle, Louise ;

Ibrahim, Mohamed ;

Gul, Sheraz ;

Fransson, Thomas ;

Brewster, Aaron S. ;

Alonso-Mori, Roberto ;

Hussein, Rana ;

Zhang, Miao ;

Douthit, Lacey ;

de Lichtenberg, Casper ;

Cheah, Mun Hon ;

Shevela, Dmitry ;

Wersig, Julia ;

Seuffert, Ina ;

Sokaras, Dimosthenis ;

Pastor, Ernest ;

Weninger, Clemens ;

Kroll, Thomas ;

Sierra, Raymond G. ;

Aller, Pierre ;

Butryn, Agata ;

Orville, Allen M. ;

Liang, Mengning ;

Batyuk, Alexander ;

Koglin, Jason E. ;

Carbajo, Sergio ;

Boutet, Sebastien ;

Moriarty, Nigel W. ;

Holton, James M. ;

Dobbek, Holger ;

Adams, Paul D. ;

Bergmann, Uwe ;

Sauter, Nicholas K. ;

Zouni, Athina ;

Messinger, Johannes ;

Yano, Junko ;

Yachandra, Vittal K. .

NATURE, 2018, 563 (7731) :421-+

[65] Confinement in crystal lattice alters entire photocycle pathway of the Photoactive Yellow Protein [J].

Konold, Patrick E. ;

Arik, Enis ;

Weissenborn, Jorn ;

Arents, Jos C. ;

Hellingwerf, Klaas J. ;

van Stokkum, Ivo H. M. ;

Kennis, John T. M. ;

Groot, Marie Louise .

NATURE COMMUNICATIONS, 2020, 11 (01)

[66] Initial events in the photocycle of photoactive yellow protein [J].

Kort, R ;

Hellingwerf, KJ ;

Ravelli, RBG .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2004, 279 (25) :26417-26424

[67] Three-dimensional view of ultrafast dynamics in photoexcited bacteriorhodopsin [J].

Kovacs, Gabriela Nass ;

Colletier, Jacques-Philippe ;

Gruenbein, Marie Luise ;

Yang, Yang ;

Stensitzki, Till ;

Batyuk, Alexander ;

Carbajo, Sergio ;

Doak, R. Bruce ;

Ehrenberg, David ;

Foucar, Lutz ;

Gasper, Raphael ;

Gorel, Alexander ;

Hilpert, Mario ;

Kloos, Marco ;

Koglin, Jason E. ;

Reinstein, Jochen ;

Roome, Christopher M. ;

Schlesinger, Ramona ;

Seaberg, Matthew ;

Shoeman, Obert L. ;

Stricker, Miriam ;

Boutet, Sebastien ;

Haacke, Stefan ;

Heberle, Joachim ;

Heyne, Karsten ;

Domratcheva, Tatiana ;

Barends, Thomas R. M. ;

Schlichting, Ilme .

NATURE COMMUNICATIONS, 2019, 10 (1)

[68] Mechanisms of inward transmembrane proton translocation [J].

Kovalev, Kirill ;

Tsybrov, Fedor ;

Alekseev, Alexey ;

Shevchenko, Vitaly ;

Soloviov, Dmytro ;

Siletsky, Sergey ;

Bourenkov, Gleb ;

Agthe, Michael ;

Nikolova, Marina ;

von Stetten, David ;

Astashkin, Roman ;

Bukhdruker, Sergey ;

Chizhov, Igor ;

Royant, Antoine ;

Kuzmin, Alexander ;

Gushchin, Ivan ;

Rosselli, Riccardo ;

Rodriguez-Valera, Francisco ;

Ilyinskiy, Nikolay ;

Rogachev, Andrey ;

Borshchevskiy, Valentin ;

Schneider, Thomas R. ;

Bamberg, Ernst ;

Gordeliy, Valentin .

NATURE STRUCTURAL & MOLECULAR BIOLOGY, 2023, 30 (07) :970-+

[69] Molecular mechanism of light-driven sodium pumping [J].

Kovalev, Kirill ;

Astashkin, Roman ;

Gushchin, Ivan ;

Orekhov, Philipp ;

Volkov, Dmytro ;

Zinovev, Egor ;

Marin, Egor ;

Rulev, Maksim ;

Alekseev, Alexey ;

Royant, Antoine ;

Carpentier, Philippe ;

Vaganova, Svetlana ;

Zabelskii, Dmitrii ;

Baeken, Christian ;

Sergeev, Ilya ;

Balandin, Taras ;

Bourenkov, Gleb ;

Carpena, Xavier ;

Boer, Roeland ;

Maliar, Nina ;

Borshchevskiy, Valentin ;

Bueldt, Georg ;

Bamberg, Ernst ;

Gordeliy, Valentin .

NATURE COMMUNICATIONS, 2020, 11 (01)

[70] Bacteriorhodopsin -: the movie [J].

Kühlbrandt, W .

NATURE, 2000, 406 (6796) :569-570

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