Screening of subclinical functional hemoglobin and red blood cell abnormalities among blood donors of Fayoum University Hospital in Egypt: Are RET-He, and IRF useful screening tools?

被引:1
作者
Bakr, Salwa [1 ,5 ]
Yousief, Eman [1 ]
Ezzat, Eman Mahmoud [2 ]
Elsary, Asmaa Younis [3 ]
Elamir, Azza M. [4 ]
Gamal, Mona [1 ]
机构
[1] Fayoum Univ, Fac Med, Dept Clin Pathol Hematol, Faiyum, Egypt
[2] Fayoum Univ, Fac Med, Dept Internal Med, Faiyum, Egypt
[3] Fayoum Univ, Fac Med, Dept Publ Hlth & Community Med, Faiyum, Egypt
[4] Fayoum Univ, Fac Med, Dept Med Biochem & Mol Biol, Faiyum, Egypt
[5] Fayoum Univ, Fac Med, Dept Clin Pathol Hematol & Transfus Med, POB 63514, Al Fayyum, Egypt
关键词
Blood donor; Red cell screening; Neonatal transfusion; Transfusion-dependent; RET-He; IRF; IRON-DEFICIENCY ANEMIA; BETA-THALASSEMIA TRAIT; GLUCOSE-6-PHOSPHATE-DEHYDROGENASE DEFICIENCY; DIAGNOSIS; TRANSFUSION; PREVALENCE; STORAGE; DIFFERENTIATE; PARAMETERS; EQUIVALENT;
D O I
10.1016/j.transci.2023.103781
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Background: The effectiveness of red cell transfusion in a given blood unit that relied on both quantity and quality of donated cells undoubtedly affects prognostic outcomes.Objective: We aimed to determine the frequency of subclinical functional hemoglobin and red cell abnormalities in donated blood of Fayoum University Hospital in Egypt. Additionally, to assess the usefulness of reticulocyte mean hemoglobin content (RET-He) and immature reticulocyte fraction (IRF) as screening measures for such abnormalities.Material and methods: This cross-sectional study enrolled 200 volunteer blood donors who met the national standard criterion of blood donation. Complete blood count with reticulocyte parameters, serum ferritin, sickling test, G6PD assay, Mentzer index, and naked-eye single tube red cell osmotic fragility test were carried out.Results: Functional red cell abnormalities represented 44 % of this cohort. Out of them, 4.5 % had iron deficiency, 11 % had a positive sickling test, 19 % had G6PD deficiency, and 9.5 % had suspicious thalassemia. The sensitivity and specificity test for RET-He in selective identification of functional hemoglobin abnormalities in donated blood were 83.3 % and 61.2 %, respectively at a cutoff value of 26.9. Though there was no statistically significant effect of RET-He on the selective detection of G6PD deficiency, IRF had a statistically significant high level with a p-value of 0.04.Conclusion: Subclinical functional red cell abnormalities seem to be prevalent among blood donors. Reticulocyte/ erythrocyte indices could be useful screening tools for red cell abnormalities. Further studies are required for assessing the impact of transfusing such abnormalities to neonates and other critical recipients.
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页数:7
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