Novel Hypocholesterolemic Peptides Derived from Silver Carp Muscle: The Modulatory Effects on Enterohepatic Cholesterol Metabolism In Vitro and In Vivo

被引：6
作者：
Wang, Kai [3 ]
Han, Lihua [3 ]
Tan, Yuqing [3 ]
Hong, Hui [3 ]
Fan, Hongbing [4 ]
Luo, Yongkang [1 ,2 ]
机构：
[1] China Agr Univ, Coll Food Sci & Nutr Engn, Beijing Lab Food Qual & Safety, Beijing 100083, Peoples R China
[2] Jiangxi Normal Univ, Natl Res & Dev Ctr Freshwater Fish Proc, Nanchang 330022, Jiangxi, Peoples R China
[3] China Agr Univ, Coll Food Sci & Nutr Engn, Beijing Lab Food Qual & Safety, Beijing 100083, Peoples R China
[4] Univ Kentucky, Dept Anim & Food Sci, Lexington, KY 40546 USA
来源：
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2023年 / 71卷 / 14期
关键词：
silver carp muscle hydrolysate; cholesterol absorption; LDL uptake; hypercholesterolemia mice; transepithelial transport; peptide sequences; SIMULATED GASTROINTESTINAL DIGESTION; ENZYME-INHIBITORY PEPTIDES; HEPG2; CELLS; PROTEIN; EXPRESSION; PCSK9; IDENTIFICATION; REGULATORS; STEROL; NPC1L1;
D O I：
10.1021/acs.jafc.2c09092
中图分类号：
S [农业科学];
学科分类号：
09 ;
摘要：
This research aimed to investigate the effect of silver carp hydrolysates (SCHs) on hypercholesterolemia and enterohepatic cholesterol metabolism. Results showed that in vitro gastrointestinal digestion products of Alcalase-SCH (GID-Alcalase) exhibited the highest inhibitory activity of cholesterol absorption mainly through downregulating the expression of essential genes related to cholesterol transport in a Caco-2 monolayer. After being absorbed by the Caco-2 monolayer, GID-Alcalase increased the low-density lipoprotein (LDL) uptake in HepG2 cells by enhancing the protein level of the LDL receptor (LDLR). The in vivo experiment showed that long-term intervention of Alcalase-SCH ameliorated hypercholesterolemia in ApoE-/- mice fed with a Western diet (WD). After transepithelial transport, four novel peptides (TKY, LIL, FPK, and IAIM) were identified, and these peptides possessed dual hypocholesterolemic functions including inhibition of cholesterol absorption and promotion of peripheric LDL uptake. Our results indicated for the first time the potential of SCHs as functional food ingredients for the management of hypercholesterolemia.
引用
收藏
页码：5565 / 5575
页数：11
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