Microplastics enhance the toxicity and phototoxicity of UV filter avobenzone on Daphnia magna

Microplastics (MPs) and ultraviolet (UV) filters cause pollution in aquatic ecosystems. Moreover, regulations on the use and discharge of UV filters in personal care products are lacking. Therefore, the combined toxicity of MPs (virgin polystyrene (PS) spheres; size: 200 nm) and avobenzone (AVO; a UV filter) on Daphnia magna were assessed. The exposure groups were AVO, AVO + UV irradiation for 6 h [AVO (UV)], AVO with MPs (Mix), and AVO with MPs + UV irradiation for 6 h [Mix (UV)]. The daphnids were exposed to these treatments for 48 h and observed for an additional 6 h. Energy reserves of all treated groups increased compared to that of the control group. Growth in the Mix group was inhibited despite a high food uptake, and food uptake and growth inhibition were validated in the Mix (UV) group. Additionally, the food uptake of the AVO (UV) and Mix (UV) groups decreased during the recovery period, possibly owing to a decrease in the normal feeding ability resulting from an increase in abnormality. These results indicate that the combined toxicity of MPs+AVO can be exacerbated under natural conditions; the complex toxicity should be considered when assessing aquatic environment pollution.