An ultra-sensitive ESIPT fluorescent probe for distinguishing cancerous cells and diagnosing APAP-induced liver injury via HClO fluctuation

被引：35
作者：
Chen, Miao [1 ,2 ,3 ]
Lin, Shuiling [1 ,2 ,3 ]
Chen, Xiaoyu [1 ,2 ,3 ]
Wang, Yu [1 ,2 ,3 ]
Zhang, Jian [4 ]
Chen, Tongsheng [1 ,2 ,3 ]
机构：
[1] South China Normal Univ, Coll Biophoton, MOE Key Lab Laser Life Sci, Guangzhou 510631, Peoples R China
[2] South China Normal Univ, Coll Biophoton, Inst Laser Life Sci, Guangzhou 510631, Peoples R China
[3] South China Normal Univ, Coll Biophoton, Guangdong Prov Key Lab Laser Life Sci, Guangzhou 510631, Peoples R China
[4] Henan Univ, Sch Mat Sci & Engn, Key Lab Special Funct Mat, Minist Educ, Kaifeng 475004, Peoples R China
来源：
SENSORS AND ACTUATORS B-CHEMICAL | 2023年 / 386卷
关键词：
HClO; Fluorescent probe; Cancerous cells distinguishing; Liver injury diagnosing; LARGE STOKES SHIFT; HYPOCHLOROUS-ACID; OXIDATIVE STRESS; REACTIVE OXYGEN; LIVING CELLS; ROS;
D O I：
10.1016/j.snb.2023.133749
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Hypochlorous acid (HClO) has been gradually recognized as a significant reactive oxygen species (ROS) owing to its stability in organisms. ROS concentration in cancerous cells is approximately ten times that in normal cells and excessive HClO is closely associated with tissue damage. Herein, a new turn-on fluorescent probe (MOBT-Cl) based on ESIPT for sensing HClO has been synthesized. MOBT-Cl possessed a large Stokes shift (130 nm), a rapid response time (around 10 s), and ultra-sensitivity (110-folds, LOD = 0.14 nM). Moreover, MOBT-Cl was suc-cessfully applied to imaging HClO in cells, zebrafish, and mice. Importantly, MOBT-Cl effectively distinguished cancerous cells from normal cells and monitored HClO levels in APAP-treated cells and zebrafish as well as diagnosed APAP-induced liver injury. The proposed probe held great potential for investigating the precise role of HClO in multiple physiological processes.
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页数：8
相关论文
共 57 条
[1]   Oxidative stress in neurodegeneration: cause or consequence? [J].
Andersen, JK .
NATURE MEDICINE, 2004, 10 (07) :S18-S25
[2]   An ESIPT fluorescent probe for ultrarapid HClO detection during reagent-stimulated oxidative stress in cells and zebrafish [J].
Chen, Miao ;
Chen, Xiaoyu ;
Wang, Yu ;
Fan, Xuhong ;
Chen, Tongsheng ;
Liang, Zhenhao .
SENSORS AND ACTUATORS B-CHEMICAL, 2022, 371
[3]   A ratiometric ESIPT fluorescent probe for detection of anticancer-associated H2O2 level in vitro and in vivo [J].
Chen, Miao ;
Liang, Zhenhao ;
Fan, Xuhong ;
Qu, Rumeng ;
Wang, Huanhuan ;
Chen, Tongsheng .
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY, 2022, 276
[4]   An ESIPT-based NIR-emitting ratiometric fluorescent probe for monitoring hydrogen peroxide in living cells and zebrafish [J].
Chen, Miao ;
Liang, Zhenhao ;
Zeng, Guanling ;
Wang, Yu ;
Mai, Zihao ;
Chen, Xiaoyu ;
Wu, Ge ;
Chen, Tongsheng .
DYES AND PIGMENTS, 2022, 198
[5]   Homopropargyl as a new recognition moiety of a fluorescent probe for detection of palladium in living cells [J].
Chen, Miao ;
Wang, Xianhui ;
Wang, Nannan ;
Ji, Xin ;
He, Jinling ;
Zhang, Jian ;
Zhao, Weili .
ANALYTICAL METHODS, 2019, 11 (32) :4093-4098
[6]   A near-infrared fluorescent probe for selective detection of HClO based on Se-sensitized aggregation of heptamethine cyanine dye [J].
Cheng, Guanghui ;
Fan, Jiangli ;
Sun, Wen ;
Cao, Jianfang ;
Hu, Chong ;
Peng, Xiaojun .
CHEMICAL COMMUNICATIONS, 2014, 50 (08) :1018-1020
[7]   The role of ROS in tumour development and progression [J].
Cheung, Eric C. ;
Vousden, Karen H. .
NATURE REVIEWS CANCER, 2022, 22 (05) :280-297
[8]   BODIPY-based near-infrared fluorescent probe for diagnosis drug-induced liver injury via imaging of HClO in cells and in vivo [J].
Fan, Guanwen ;
Wang, Nannan ;
Zhang, Jian ;
Ji, Xin ;
Qin, Shuchun ;
Tao, Yuanfang ;
Zhao, Weili .
DYES AND PIGMENTS, 2022, 199
[9]   Antimicrobial reactive oxygen and nitrogen species: Concepts and controversies [J].
Fang, FC .
NATURE REVIEWS MICROBIOLOGY, 2004, 2 (10) :820-832
[10]   Hypochlorous acid is antipruritic and anti-inflammatory in a mouse model of atopic dermatitis [J].
Fukuyama, T. ;
Martel, B. C. ;
Linder, K. E. ;
Ehling, S. ;
Ganchingco, J. R. ;
Baeumer, W. .
CLINICAL AND EXPERIMENTAL ALLERGY, 2018, 48 (01) :78-88
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