The orchestration of sustained drug delivery by bacterial cellulose/gelatin nanocomposites reinforced with carboxylic carbon nanotubes

Recently, modifying bacterial cellulose (BC) by compositing it with other nano-biomaterials has become inevitable to achieve its desired properties in drug delivery. To address this, our study endeavors to utilize an in-situ fabrication method for the creation of a multifunctional BC/gelatin (BC/Gel) platform reinforced with carboxylic multi-walled carbon nanotubes (cMWCNTs) as a sustainable delivery model of biomolecules. Incipiently, cMWCNTs were loaded with human serum albumin (HSA) as a drug model, with an optimized nanoparticle-to-protein ratio of 1:5 and loading efficiency of 90.0 +/- 1.0 % before incorporation into BC/Gel hydrogels. By comparison, nanocomposition improved the surface area and overall porosity of BC/Gel up to 58.0 +/- 1.3 m(2)/g and 85.5 +/- 1.1 %, respectively. Likewise, significant wettability of 44.0 +/- 0.1 degrees and dramatic biodegradation rate of 36.9 +/- 1.2 % were other exceptionally gained attributes. Meanwhile, with a Zero-order kinetic mechanism, CNT-HSA integration facilitated the controlled release of 56.0 +/- 0.9 % HSA over 7 days. Drug-loaded nanocomposites showcased >70 % viability during in vitro cellular trials using Human Foreskin Fibroblasts (HFF). Overall, BC/Gel/CNT-HSA nanocomposite exhibited favorable cell behavior, devoid of cytotoxic manifestations. Consequently, this BC-based nanocomposite scaffold implicates the premiere capability in the sustained delivery of an extended range of protein biomolecules, offering a promising therapeutic avenue for bolstering tissue regeneration.