Circadian clock gene BMAL1 regulates STAR expression in goose ovarian preovulatory granulosa cells

被引：5

作者：

Chen, Rong ^{[1
,2
]}

Qin, Yifei ^{[1
,2
]}

Du, Jie ^{[3
]}

Liu, Jie ^{[1
,2
]}

Dai, Shudi ^{[1
,2
]}

Lei, Mingming ^{[1
,2
]}

Zhu, Huanxi ^{[1
,2
]}

机构：

[1] Minist Agr & Rural Affairs, Key Lab Crop & Anim Integrated Farming, Nanjing 210014, Peoples R China

[2] Jiangsu Acad Agr Sci, Inst Anim Sci, Nanjing, Peoples R China

[3] Jiangsu Vocat Coll Agr & Forestry, Anim Husb & Vet Coll, Jurong, Peoples R China

来源：

POULTRY SCIENCE | 2023年 / 102卷 / 12期

关键词：

goose; circadian clock; BMAL1; STAR; granulosa cell; MESSENGER-RIBONUCLEIC-ACID; LUTEINIZING-HORMONE; PROGESTERONE PRODUCTION; HEN;

D O I：

10.1016/j.psj.2023.103159

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

The ovarian circadian clock plays a regulatory role in the avian ovulation-oviposition cycle. How-ever, little is known regarding the ovarian circadian clock of geese. In this study, we investigated rhythmic changes in clock genes over a 48-h period and identified potential clock-controlled genes involved in progesterone synthesis in goose ovarian preovulatory granulosa cells. The results showed that BMAL1, CRY1, and CRY2, as well as 4 genes (LHR, STAR, CYP11A1, and HSD3B) involved in progesterone synthesis exhibited rhythmic expression patterns in goose ovarian preovulatory granulosa cells over a 48-h period. Knockdown of BMAL1decreased the progesterone concentration and downregulated STAR mRNA and protein levels in goose ovarian preovulatory granulosa cells. Overexpression of BMAL1 increased the progesterone concentration and upregulated the STAR mRNA level in goose ovarian preovulatory granulosa cells. Moreover, we demonstrated that the BMAL1/CLOCK complex activated the transcription of goose STAR gene by binding to an E-box motif. These results suggest that the circadian clock is involved in the regulation of progesterone synthesis in goose ovarian preovulatory granulosa cells by orchestrating the transcription of steroidogenesis-related genes.

引用

页数：8

共 35 条

[31] Timing of the Ovarian Circadian Clock Is Regulated by Gonadotropins [J].

Yoshikawa, Tomoko ;

Sellix, Michael ;

Pezuk, Pinar ;

Menaker, Michael .

ENDOCRINOLOGY, 2009, 150 (09) :4338-4347

[32] Reducing light exposure enhances the circadian rhythm of the biological clock through interactions with the gut microbiota [J].

Zhang, Yu ;

Li, Yong ;

Yuan, Yilin ;

Wang, Jun ;

Zhang, Shiyu ;

Zhu, Run ;

Wang, Yan ;

Wu, Yinbao ;

Liao, Xindi ;

Mi, Jiandui .

SCIENCE OF THE TOTAL ENVIRONMENT, 2023, 858

[33] Circadian clock genes are rhythmically expressed in specific segments of the hen oviduct [J].

Zhang, Z. C. ;

Wang, Y. G. ;

Li, L. ;

Yin, H. D. ;

Li, D. Y. ;

Wang, Y. ;

Zhao, X. L. ;

Liu, Y. P. ;

Zhu, Q. .

POULTRY SCIENCE, 2016, 95 (07) :1653-1659

[34] A transcriptome analysis for 24-hour continuous sampled uterus reveals circadian regulation of the key pathways involved in eggshell formation of chicken [J].

Zhang, Zhichao ;

Du, Xiaxia ;

Lai, Shuang ;

Shu, Gang ;

Zhu, Qing ;

Tian, Yaofu ;

Li, Diyan ;

Wang, Yan ;

Yang, Jiandong ;

Zhang, Yao ;

Zhao, Xiaoling .

POULTRY SCIENCE, 2022, 101 (01)

[35] Rhythmic expression of circadian clock genes in the preovulatory ovarian follicles of the laying hen [J].

Zhang, Zhichao ;

Lai, Shuang ;

Wang, Yagang ;

Li, Liang ;

Yin, Huadong ;

Wang, Yan ;

Zhao, Xiaoling ;

Li, Diyan ;

Yang, Mingyao ;

Zhu, Qing .

PLOS ONE, 2017, 12 (06)

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