Protein-Protein Interaction in Multicomponent Reaction Enables Chemoselective, Site-Selective, and Modular Labeling of Native Proteins

被引：9

作者：

Molla, Rajib ^{[1
]}

Joshi, Pralhad N. ^{[1
]}

Reddy, Neelesh C. ^{[1
]}

Biswas, Dwaipayan ^{[1
]}

Rai, Vishal ^{[1
]}

机构：

[1] Indian Inst Sci Educ & Res IISER Bhopal, Dept Chem, Bhopal Bypass Rd, Bhopal 462066, MP, India

来源：

ORGANIC LETTERS | 2023年 / 25卷 / 34期

关键词：

CHEMISTRY;

D O I：

10.1021/acs.orglett.3c02405

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

A protein's pool of functionalities presents aformidablechallenge for its single-site modification. Here, we report a methodto harness protein-protein interaction (PPI) to drive selectivemodification. It involves the chemoselective reversible generationof reactive intermediates and utilizes PPI-specificity to drive thesubsequent site-selective irreversible step. The disintegrate (DIN) theory-driven multicomponent aza-Morita-Baylis-Hillman(aza-MBH) reaction offers homogeneous and modular single-site proteinmodification capable of late-stage mono- and dual-probe installation.

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收藏

页码：6385 / 6390

页数：6

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