Development and Validation of Analytical Method by HPLC-PDA and Seasonality from Gallic Acid, Catechin, and Epicatechin in Leaf Extracts from Campomanesia adamantium

被引:1
作者
Cabral, Raphael O. [1 ]
Borges, Leonardo L. [2 ,3 ]
de Oliveira, Matheus G. [1 ]
Romano, Camila A. [1 ]
Fiuza, Tatiana S. [1 ,4 ]
de Paula, Jose R. [1 ]
机构
[1] Univ Fed Goias, Fac Farm, BR-74605170 Goiania, GO, Brazil
[2] Univ Estadual Goias, Campus Cent, BR-75132903 Anapolis, GO, Brazil
[3] Pontificia Univ Catolica Goias, Escola Ciencias Med & Vida, BR-74605050 Goiania, GO, Brazil
[4] Univ Fed Goias, Inst Ciencias Biol, BR-74690900 Goiania, GO, Brazil
关键词
Cerrado; chemical markers; gabiroba; quality control; seasonal variability; ultrasound-assisted extraction; PHENOLIC-COMPOUNDS; ANTIOXIDANT; MYRTACEAE; TANNINS; SYSTEM; GREEN;
D O I
10.21577/0103-5053.20230147
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Campomanesia adamantium is native of the Brazilian Cerrado, and its leaves are popularly used as anti-inflammatory, antidiarrheal, and antiseptic in the urinary tract. This study aimed to develop and validate an analytical method by high-performance liquid chromatography for the simultaneous quantification and evaluation of the seasonal variability of gallic acid, catechin, and epicatechin, during 12 months, in C. adamantium leaf extracts. Chromatographic separations were performed with a mobile phase gradient of acetonitrile and 0.05% (v/v) trifluoroacetic acid solution, flow rate of 1.0 mL min-1, detection at 210 nm, C18 column (150 x 4.6 mm, 5 & mu;m) and column oven temperature of 35 & DEG;C. The analytical method developed was selective, linear, precise, accurate, robust, and without matrix effect. The parameters obtained in the present study meet the requirements established by national and international guidelines. The best time for leaves harvesting, with the highest levels reached, was in September (0.0626%, m/v) and August (0.044%, m/v) for gallic acid, in June (0.3953%, m/v) and July (0.3804%, m/v) for catechin, and from May (0.1622%, m/v) and June (0.1415%, m/v) for epicatechin, in the dry season. Therefore, this study contributes by providing parameters for quality control of the raw material C. adamantium.
引用
收藏
页码:1 / 15
页数:15
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