CRISPR-Cas-Based Antimicrobials: Design, Challenges, and Bacterial Mechanisms of Resistance

被引:34
|
作者
Mayorga-Ramos, Arianna [1 ]
Zuniga-Miranda, Johana [1 ]
Carrera-Pacheco, Saskya E. [1 ]
Barba-Ostria, Carlos [2 ]
Guaman, Linda P. [1 ]
机构
[1] Univ UTE, Fac Ciencias Salud Eugenio Espejo, Ctr Invest Biomed CENBIO, Quito 170527, Ecuador
[2] USFQ, Escuela Med, Colegio Ciencias Salud Quito, Quito 170902, Ecuador
来源
ACS INFECTIOUS DISEASES | 2023年 / 9卷 / 07期
关键词
CRISPR-Cas; antimicrobial design; antibioticresistance; SEQUENCE-SPECIFIC ANTIMICROBIALS; PSEUDOMONAS-AERUGINOSA BIOFILMS; BACTERIOPHAGE HOST-RANGE; HORIZONTAL GENE-TRANSFER; ESCHERICHIA-COLI; PLASMID TRANSFER; PHAGE THERAPY; DNA CLEAVAGE; ANTI-CRISPR; DELIVERY;
D O I
10.1021/acsinfecdis.2c00649
中图分类号
R914 [药物化学];
学科分类号
100701 ;
摘要
The emergence of antibiotic-resistant bacterial strainsis a sourceof public health concern across the globe. As the discovery of newconventional antibiotics has stalled significantly over the past decade,there is an urgency to develop novel approaches to address drug resistancein infectious diseases. The use of a CRISPR-Cas-based system for theprecise elimination of targeted bacterial populations holds promiseas an innovative approach for new antimicrobial agent design. TheCRISPR-Cas targeting system is celebrated for its high versatilityand specificity, offering an excellent opportunity to fight antibioticresistance in pathogens by selectively inactivating genes involvedin antibiotic resistance, biofilm formation, pathogenicity, virulence,or bacterial viability. The CRISPR-Cas strategy can enact antimicrobialeffects by two approaches: inactivation of chromosomal genes or curingof plasmids encoding antibiotic resistance. In this Review, we providean overview of the main CRISPR-Cas systems utilized for the creationof these antimicrobials, as well as highlighting promising studiesin the field. We also offer a detailed discussion about the most commonlyused mechanisms for CRISPR-Cas delivery: bacteriophages, nanoparticles,and conjugative plasmids. Lastly, we address possible mechanisms ofinterference that should be considered during the intelligent designof these novel approaches.
引用
收藏
页码:1283 / 1302
页数:20
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