Myokine musclin alleviates lipid accumulation in 3T3-L1 adipocytes through PKA/p38-mediated upregulation of lipolysis and suppression of lipogenesis

被引：6

作者：

Choi, Sung Woo ^{[1
]}

Oh, Heeseung ^{[1
]}

Park, Seung Yeon ^{[1
,2
]}

Cho, Wonjun ^{[1
]}

Abd El-Aty, A. M. ^{[3
,4
]}

Hacimuftuoglu, Ahmet ^{[4
,5
]}

Jeong, Ji Hoon ^{[1
,2
]}

Jung, Tae Woo ^{[1
,6
]}

机构：

[1] Chung Ang Univ, Coll Med, Dept Pharmacol, Seoul, South Korea

[2] Ang Univ, Grad Sch Chung, Dept Global Innovat Drugs, Seoul, South Korea

[3] Cairo Univ, Fac Vet Med, Dept Pharmacol, Giza 12211, Egypt

[4] Ataturk Univ, Med Fac, Dept Med Pharmacol, TR-25240 Erzurum, Turkey

[5] Ataturk Univ, Vaccine Dev Applicat & Res Ctr, TR-25240 Erzurum, Turkey

[6] Chung Ang Univ, Coll Med, Dept Pharmacol, 221 Heuksuk Dong, Seoul 156756, South Korea

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2023年 / 642卷

基金：

新加坡国家研究基金会;

关键词：

Musclin; Myokine; Adipocyte; Obesity; Lipogenesis; Lipolysis; SKELETAL-MUSCLE; PROTEIN; DIFFERENTIATION; RESISTANCE; OSTEOCRIN; EXERTS; FAT;

D O I：

10.1016/j.bbrc.2022.12.056

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Musclin (MUS), an exercise-responsive myokine, has been documented to attenuate inflammation and enhance physical endurance. However, the effects of MUS on differentiation and related molecular mechanisms in adipocytes have not yet been studied. In this study, we found that treatment with MUS attenuated lipid accumulation in fully differentiated 3T3-L1 cells. Furthermore, MUS treatment enhanced lipolysis assessed by glycerol release, and caused apoptosis, whereas it reduced the expression of lipo-genic proteins, such as PPARg and processed SREBP1. Treatment with MUS augmented phosphorylated PKA expression, whereas suppressed p38 phosphorylation in 3T3-L1 adipocytes. H89, a selective PKA inhibitor reduced the effects of MUS on lipogenic lipid accumulation as well as lipolysis except for apoptosis. These results suggest that MUS promotes lipolysis and suppresses lipogenesis through a PKA/ p38-dependent pathway, thereby ameliorating lipid deposition in cultured adipocytes. The current study offers the potential of MUS as a therapeutic approach for treating obesity with few side effects.(c) 2022 Elsevier Inc. All rights reserved.

引用

页码：113 / 117

页数：5