Functionalized screen-printed electrodes for the thermal detection of Escherichia coli in dairy products

被引：14

作者：

Arreguin-Campos, Rocio ^{[1
]}

Frigoli, Margaux ^{[1
]}

Caldara, Manlio ^{[1
]}

Crapnell, Robert D. ^{[2
]}

Ferrari, Alejandro Garcia -Miranda ^{[2
]}

Banks, Craig E. ^{[2
]}

Cleij, Thomas J. ^{[1
]}

Dilie, Hanne ^{[1
]}

Eersels, Kasper ^{[1
]}

van Grinsven, Bart ^{[1
]}

机构：

[1] Maastricht Univ, Fac Sci & Engn, Sensor Engn Dept, POB 616, NL-6200 Maastricht, Netherlands

[2] Manchester Metropolitan Univ, Fac Sci & Engn, Manchester M1 5GD, England

来源：

FOOD CHEMISTRY | 2023年 / 404卷

关键词：

Imprinted polymers; Biomimetic sensing; Food monitoring; Screen-printed electrodes; LABEL-FREE DETECTION; AMPLIFICATION; APTASENSOR; O157H7; ASSAY;

D O I：

10.1016/j.foodchem.2022.134653

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Accurate and fast on-site detection of harmful microorganisms in food products is a key preventive step to avoid food-borne illness and product recall. In this study, screen-printed electrodes (SPEs) were functionalized via a facile strategy with surface imprinted polymers (SIPs). The SIP-coated SPEs were used in combination with the heat transfer method (HTM) for the real-time detection of Escherichia coli. The sensor was tested in buffer, with a reproducible and sensitive response that attained a limit of detection of 180 CFU/mL. Furthermore, selectivity was assessed by analyzing the sensor's response to C. sakazakii, K. pneumoniae and S. aureus as analogue strains. Finally, the device was successfully used for the detection of E. coli in spiked milk as proof-of-application, requiring no additional sample preparation. These results suggest the proposed thermal biosensor possesses the potential of becoming a tool for routine, on-site monitoring of E. coli in food safety applications.

引用

页数：7

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