Magnesium implant degradation provides immunomodulatory and proangiogenic effects and attenuates peri-implant fibrosis in soft tissues

被引：28

作者：

Ben Amara, Heithem ^{[1
]}

Martinez, Diana C. ^{[2
]}

Shah, Furqan A. ^{[1
]}

Loo, Anna Johansson ^{[1
]}

Emanuelsson, Lena ^{[1
]}

Norlindh, Birgitta ^{[1
]}

Willumeit-Romer, Regine ^{[3
]}

Plocinski, Tomasz ^{[2
]}

Swieszkowski, Wojciech ^{[2
]}

Palmquist, Anders ^{[1
]}

Omar, Omar ^{[1
]}

Thomsen, Peter ^{[1
]}

机构：

[1] Univ Gothenburg, Sahlgrenska Acad, Inst Clin Sci, Dept Biomat, Box 412, SE-40530 Gothenburg, Sweden

[2] Warsaw Univ Technol, Fac Mat Sci & Engn, Mat Design Div, Biomat Grp, Warsaw, Poland

[3] Helmholtz Zentrum Hereon, Inst Met Biomat, Geesthacht, Germany

来源：

BIOACTIVE MATERIALS | 2023年 / 26卷

基金：

瑞典研究理事会;

关键词：

Magnesium; Biodegradable implants; Inflammation; Foreign -body reaction; Gene expression; Neovascularization; VIVO CELLULAR INTERACTIONS; IN-VIVO; TITANIUM; ACTIVATION; CHEMOTAXIS; SECRETION; SCAFFOLDS; HYDROGEN; CELLS; SCREW;

D O I：

10.1016/j.bioactmat.2023.02.014

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Implants made of magnesium (Mg) are increasingly employed in patients to achieve osteosynthesis while degrading in situ. Since Mg implants and Mg2+ have been suggested to possess anti-inflammatory properties, the clinically observed soft tissue inflammation around Mg implants is enigmatic. Here, using a rat soft tissue model and a 1-28 d observation period, we determined the temporo-spatial cell distribution and behavior in relation to sequential changes of pure Mg implant surface properties and Mg2+ release. Compared to nondegradable titanium (Ti) implants, Mg degradation exacerbated initial inflammation. Release of Mg degradation products at the tissue-implant interface, culminating at 3 d, actively initiated chemotaxis and upregulated mRNA and protein immunomodulatory markers, particularly inducible nitric oxide synthase and toll-like receptor-4 up to 6 d, yet without a cytotoxic effect. Increased vascularization was demonstrated morphologically, preceded by high expression of vascular endothelial growth factor. The transition to appropriate tissue repair coincided with implant surface enrichment of Ca and P and reduced peri-implant Mg2+ concentration. Mg implants revealed a thinner fibrous encapsulation compared with Ti. The detailed understanding of the relationship between Mg material properties and the spatial and time-resolved cellular processes provides a basis for the interpretation of clinical observations and future tailoring of Mg implants.

引用

页码：353 / 369

页数：17

共 83 条

[81] In vivo quantification of hydrogen gas concentration in bone marrow surrounding magnesium fracture fixation hardware using an electrochemical hydrogen gas sensor [J].