Isolation and Cultivation of Adipose-Derived Mesenchymal Stem Cells Originating from the Infrapatellar Fat Pad Differentiated with Blood Products: Method and Protocol

被引：1

作者：

Neubauer, Markus ^{[1
,2
]}

Kramer, Karina ^{[1
]}

Neugebauer, Johannes ^{[1
,2
]}

Moser, Lukas ^{[1
,2
]}

Moser, Anna ^{[1
,2
]}

Dammerer, Dietmar ^{[1
,2
]}

Nehrer, Stefan ^{[1
,2
]}

机构：

[1] Danube Univ Krems, Ctr Regenerat Med & Orthoped, Dr Karl Dorrek Str 30, A-3500 Krems, Austria

[2] Karl Landsteiner Univ Hlth Sci, Univ Hosp Krems, Dept Orthoped & Traumatol, Mitterweg 10, A-3500 Krems, Austria

来源：

METHODS AND PROTOCOLS | 2023年 / 6卷 / 01期

关键词：

mesenchymal-stem-cells; adipose-tissue; infra-patellar-fat pad; blood products; joint preservation; regenerative orthopedics; STROMAL CELLS;

D O I：

10.3390/mps6010003

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Adipose-derived mesenchymal stem cells (ASCs) are a promising source for clinical application in regenerative orthopedics. ASCs derived from the infra-patellar fat pad (IFP)-a distinct adipose structure in the knee-show superior regenerative potential compared to subcutaneous-fat-derived cells. Furthermore, it has been shown that blood products enhance ASCs' viability. A major challenge for clinical translation of both ASCs and blood products is the low comparability of obtained data due to non-standardized harvesting, isolation and preparation methods. The aim of this method-paper is to provide reproducible protocols to help standardize basic research in the field to build a sound basis for clinical translation with an emphasize on practicability. The presented protocols include (i) ASC isolation from the IFP, (ii) blood product preparation and (iii) ASC incubation with blood products.

引用

页数：10

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